2RD8
Human Thymidylate Synthase Stabilized in Active Conformation by R163K Mutation: Asymmetry and Reactivity of Cys195
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0032259 | biological_process | methylation |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0071897 | biological_process | DNA biosynthetic process |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005542 | molecular_function | folic acid binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005743 | cellular_component | mitochondrial inner membrane |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0032259 | biological_process | methylation |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 0071897 | biological_process | DNA biosynthetic process |
| B | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 B 616 |
| Chain | Residue |
| A | ARG50 |
| A | ARG215 |
| A | SER216 |
| A | HOH701 |
| B | ARG175 |
| B | ARG176 |
| B | HOH626 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 B 617 |
| Chain | Residue |
| B | ARG50 |
| B | ARG215 |
| B | SER216 |
| A | ARG175 |
| A | ARG176 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE BME A 655 |
| Chain | Residue |
| A | CYS195 |
| A | ASP218 |
| A | ASN226 |
| A | HOH701 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| B | ARG175-VAL203 | |
| A | ARG175-VAL203 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| B | CME195 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| B | ARG50 | |
| B | ASN226 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| B | ARG175 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| B | CME195 | |
| B | ARG215 | |
| B | HIS256 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| B | ASP218 | |
| B | ALA312 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| B | SER114 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| B | LYS287 | |
| B | LYS292 | |
| B | LYS308 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| A | ASN226 | |
| A | SER229 | |
| A | CYS195 |
| site_id | CSA2 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| A | ASP254 | |
| A | SER216 | |
| A | CYS195 | |
| A | ASP218 | |
| A | GLU87 | |
| A | HIS256 |
| site_id | CSA3 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| B | ASP254 | |
| B | SER216 | |
| B | ASP218 | |
| B | GLU87 | |
| B | HIS256 |
