Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2QPU

Sugar tongs mutant S378P in complex with acarbose

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004556	molecular_function	alpha-amylase activity
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0005983	biological_process	starch catabolic process
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0043169	molecular_function	cation binding
A	0046872	molecular_function	metal ion binding
B	0004556	molecular_function	alpha-amylase activity
B	0005509	molecular_function	calcium ion binding
B	0005576	cellular_component	extracellular region
B	0005975	biological_process	carbohydrate metabolic process
B	0005983	biological_process	starch catabolic process
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0043169	molecular_function	cation binding
B	0046872	molecular_function	metal ion binding
C	0004556	molecular_function	alpha-amylase activity
C	0005509	molecular_function	calcium ion binding
C	0005576	cellular_component	extracellular region
C	0005975	biological_process	carbohydrate metabolic process
C	0005983	biological_process	starch catabolic process
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0043169	molecular_function	cation binding
C	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Nucleophile => ECO:0000305\|PubMed:16030022, ECO:0000305\|PubMed:7901200

Chain	Residue	Details
A	ASP180
B	ASP180
C	ASP180

site_id	SWS_FT_FI2
Number of Residues	3
Details	ACT_SITE: Proton donor => ECO:0000305\|PubMed:7901200

Chain	Residue	Details
A	GLU205
B	GLU205
C	GLU205

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305\|PubMed:12906828

Chain	Residue	Details
A	HIS45
A	ARG178
B	HIS45
B	ARG178
C	HIS45
C	ARG178

site_id	SWS_FT_FI4
Number of Residues	21
Details	BINDING: BINDING => ECO:0000305\|PubMed:16030022

Chain	Residue	Details
A	TYR52
B	ASN209
B	LYS271
B	HIS290
B	GLN296
B	TRP402
C	TYR52
C	TRP207
C	ASN209
C	LYS271
C	HIS290
A	TRP207
C	GLN296
C	TRP402
A	ASN209
A	LYS271
A	HIS290
A	GLN296
A	TRP402
B	TYR52
B	TRP207

site_id	SWS_FT_FI5
Number of Residues	39
Details	BINDING: BINDING => ECO:0000269\|PubMed:12906828, ECO:0000269\|PubMed:16030022, ECO:0000269\|PubMed:17803687, ECO:0000269\|PubMed:18588886

Chain	Residue	Details
A	ASN92
A	PHE144
A	ALA147
A	ASP149
A	GLY184
B	ASN92
B	GLU109
B	THR112
B	ASP114
B	ASP118
B	ASP128
A	GLU109
B	ASP139
B	ALA142
B	ASP143
B	PHE144
B	ALA147
B	ASP149
B	GLY184
C	ASN92
C	GLU109
C	THR112
A	THR112
C	ASP114
C	ASP118
C	ASP128
C	ASP139
C	ALA142
C	ASP143
C	PHE144
C	ALA147
C	ASP149
C	GLY184
A	ASP114
A	ASP118
A	ASP128
A	ASP139
A	ALA142
A	ASP143

site_id	SWS_FT_FI6
Number of Residues	12
Details	BINDING: BINDING => ECO:0000305\|PubMed:12906828, ECO:0000305\|PubMed:16030022

Chain	Residue	Details
A	GLN227
C	GLY277
C	LYS375
C	THR392
A	GLY277
A	LYS375
A	THR392
B	GLN227
B	GLY277
B	LYS375
B	THR392
C	GLN227

site_id	SWS_FT_FI7
Number of Residues	3
Details	BINDING: BINDING => ECO:0000305\|PubMed:16030022, ECO:0000305\|PubMed:17803687

Chain	Residue	Details
A	ASP234
B	ASP234
C	ASP234

site_id	SWS_FT_FI8
Number of Residues	3
Details	BINDING: BINDING => ECO:0000305\|PubMed:12906828, ECO:0000305\|PubMed:16030022, ECO:0000305\|PubMed:17803687, ECO:0000305\|PubMed:18588886

Chain	Residue	Details
A	TYR380
B	TYR380
C	TYR380

site_id	SWS_FT_FI9
Number of Residues	3
Details	SITE: Transition state stabilizer => ECO:0000305\|PubMed:7901200

Chain	Residue	Details
A	ASP291
B	ASP291
C	ASP291

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP180
A	GLU205

site_id	CSA10
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	HIS290
A	ARG178
A	GLU205

site_id	CSA11
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP291
B	ASP180
B	HIS290
B	ARG178
B	GLU205

site_id	CSA12
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP291
C	ASP180
C	HIS290
C	ARG178
C	GLU205

site_id	CSA13
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	HIS93
A	GLU205

site_id	CSA14
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP291
B	ASP180
B	HIS93
B	GLU205

site_id	CSA15
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP291
C	ASP180
C	HIS93
C	GLU205

site_id	CSA16
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	GLU205

site_id	CSA17
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP291
B	ASP180
B	GLU205

site_id	CSA18
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP291
C	ASP180
C	GLU205

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP180
B	GLU205

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP180
C	GLU205

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP234
A	ASP180

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP234
B	ASP180

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP234
C	ASP180

site_id	CSA7
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	TRP207
A	ASP291
A	ASP180
A	GLU205

site_id	CSA8
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	TRP207
B	ASP291
B	ASP180
B	GLU205

site_id	CSA9
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	TRP207
C	ASP291
C	ASP180
C	GLU205

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)