2PXC

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAM and GTPA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE SAM A 500

Chain	Residue
A	SER56
A	GLU111
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	HOH612
A	GLY58
A	GLY81
A	CYS82
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	HIS110

---

site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE G3A A 501

Chain	Residue
A	LYS13
A	LEU16
A	ASN17
A	MET19
A	PHE24
A	ARG28
A	ARG41
A	ARG42
A	ARG45
A	SER150
A	SER151
A	PRO152
A	SER215
A	ASN216
A	G3A502

---

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE G3A A 502

Chain	Residue
A	ARG37
A	ARG41
A	ARG41
A	ARG44
A	ARG44
A	SER56
A	ARG57
A	ARG84
A	GLY109
A	GLU111
A	ARG213
A	G3A501

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS182
A	GLU218

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	SER56
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	ASP131
A	VAL132
A	ILE147
A	TYR220

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS13
A	ASN17
A	PHE24
A	ARG28
A	SER150
A	ARG213
A	SER215

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU16
A	MET19

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS182
A	GLU218

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56

---