2PX4

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	methyltransferase cap1 activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 890

Chain	Residue
A	TYR119
A	ALA266

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 925

Chain	Residue
A	GLY52
A	ASN259
A	LEU260
A	GLY261

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 894

Chain	Residue
A	GLU149
A	HOH930

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 601

Chain	Residue
A	ARG41
A	SER56
A	ARG57
A	ARG84
A	HOH979
A	ARG37

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 A 602

Chain	Residue
A	ARG57
A	LYS61
A	LEU211
A	SER212
A	ARG213
A	ASN216
A	GLU218
A	SO4606

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 603

Chain	Residue
A	ARG63
A	VAL258
A	ASN259
A	HOH959
A	HOH972

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 604

Chain	Residue
A	GLY203
A	GLY204
A	SER223
A	HOH994
A	HOH1044

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 605

Chain	Residue
A	ARG243
A	HOH917
A	HOH945
A	HOH1043

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 606

Chain	Residue
A	PHE24
A	ARG28
A	ARG213
A	SER215
A	SO4602
A	HOH949

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 607

Chain	Residue
A	ARG84
A	GLU111
A	GLU112
A	HOH920
A	HOH992

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE UNX A 801

Chain	Residue
A	ASP170
A	UNX802
A	UNX803
A	UNX806
A	HOH1007

---

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE UNX A 802

Chain	Residue
A	SER137
A	MET167
A	ASP170
A	TRP171
A	UNX801
A	UNX803
A	HOH914

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE UNX A 803

Chain	Residue
A	SER137
A	ASP170
A	UNX801
A	UNX802
A	UNX804

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE UNX A 804

Chain	Residue
A	GLU22
A	SER137
A	UNX803

---

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE UNX A 805

Chain	Residue
A	PHE133
A	UNX807
A	UNX808
A	UNX809

---

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE UNX A 806

Chain	Residue
A	ASP170
A	UNX801
A	UNX809

---

site_id	BC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE UNX A 807

Chain	Residue
A	PHE133
A	UNX805

---

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE UNX A 808

Chain	Residue
A	ARG163
A	UNX805
A	UNX809

---

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE UNX A 809

Chain	Residue
A	PHE133
A	UNX805
A	UNX806
A	UNX808

---

site_id	CC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE SAH A 500

Chain	Residue
A	HOH896
A	HOH909
A	SER56
A	GLY58
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	LYS105
A	HIS110
A	GLU111
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	HOH892

---

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 701

Chain	Residue
A	GLU22
A	GLU23
A	SER173
A	TRP249
A	HOH1031
A	HOH1036

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"For 2'-O-MTase activity","evidences":[{"source":"UniProtKB","id":"Q6YMS4","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"mRNA cap binding; via carbonyl oxygen","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Site: {"description":"Essential for 2'-O-methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Site: {"description":"Essential for 2'-O-methyltransferase and N-7 methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P03314","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---