Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2PPB

Crystal structure of the T. thermophilus RNAP polymerase elongation complex with the ntp substrate analog and antibiotic streptolydigin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0016779	molecular_function	nucleotidyltransferase activity
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
C	0016779	molecular_function	nucleotidyltransferase activity
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
D	0000287	molecular_function	magnesium ion binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0005515	molecular_function	protein binding
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0016779	molecular_function	nucleotidyltransferase activity
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0046872	molecular_function	metal ion binding
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
E	0016779	molecular_function	nucleotidyltransferase activity
E	0034062	molecular_function	5'-3' RNA polymerase activity
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0006351	biological_process	DNA-templated transcription
K	0016779	molecular_function	nucleotidyltransferase activity
K	0034062	molecular_function	5'-3' RNA polymerase activity
K	0046983	molecular_function	protein dimerization activity
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0006351	biological_process	DNA-templated transcription
L	0016779	molecular_function	nucleotidyltransferase activity
L	0034062	molecular_function	5'-3' RNA polymerase activity
L	0046983	molecular_function	protein dimerization activity
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0003677	molecular_function	DNA binding
M	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
M	0006351	biological_process	DNA-templated transcription
M	0016779	molecular_function	nucleotidyltransferase activity
M	0032549	molecular_function	ribonucleoside binding
M	0034062	molecular_function	5'-3' RNA polymerase activity
N	0000287	molecular_function	magnesium ion binding
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0003677	molecular_function	DNA binding
N	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
N	0005515	molecular_function	protein binding
N	0006351	biological_process	DNA-templated transcription
N	0008270	molecular_function	zinc ion binding
N	0016779	molecular_function	nucleotidyltransferase activity
N	0034062	molecular_function	5'-3' RNA polymerase activity
N	0046872	molecular_function	metal ion binding
O	0000428	cellular_component	DNA-directed RNA polymerase complex
O	0003677	molecular_function	DNA binding
O	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
O	0006351	biological_process	DNA-templated transcription
O	0016779	molecular_function	nucleotidyltransferase activity
O	0034062	molecular_function	5'-3' RNA polymerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE STD D 7001

Chain	Residue
C	ARG422
D	PRO1257
D	HOH9308
I	DG5
C	ARG428
C	ALA447
C	ILE449
D	ALA1082
D	ALA1085
D	LEU1086
D	ARG1087
D	ASP1090

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE STD N 8001

Chain	Residue
M	ARG422
M	PHE425
M	ALA447
M	ILE449
N	ALA1082
N	ALA1085
N	LEU1086
N	ARG1087
N	ASP1090
N	PRO1257
N	HOH9065
Z	DG5

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 7058

Chain	Residue
D	CYS58
D	CYS60
D	CYS73
D	CYS76

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 8112

Chain	Residue
D	CYS1112
D	CYS1194
D	CYS1201
D	CYS1204

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 7158

Chain	Residue
N	CYS58
N	CYS60
N	CYS73
N	CYS76

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 8212

Chain	Residue
N	CYS1112
N	CYS1194
N	CYS1201
N	CYS1204

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 9001

Chain	Residue
D	ASP739
D	ASP741
D	ASP743
H	G16

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 9002

Chain	Residue
D	ASP739
D	APC5999
D	HOH9004

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG N 9003

Chain	Residue
N	ASP739
N	ASP741
N	ASP743
Y	G16

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG N 9004

Chain	Residue
M	APC6999
M	HOH7001
N	ASP739

site_id	BC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE APC D 5999

Chain	Residue
C	ARG557
C	ARG879
D	ARG704
D	PRO706
D	ASN737
D	ARG1029
D	MG9002
D	HOH9003
D	HOH9004
D	HOH9231
D	HOH9347
D	HOH9498
G	DT14
H	G16

site_id	BC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE APC M 6999

Chain	Residue
M	ARG557
M	ARG879
M	HOH7000
M	HOH7319
N	ARG704
N	PRO706
N	ASN737
N	ARG1029
N	MG9004
X	DT14
Y	G16

Functional Information from PROSITE/UniProt

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKLANrHGNKGV

Chain	Residue	Details
C	GLY836-VAL848

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01322

Chain	Residue	Details
D	CYS58
D	CYS1201
D	CYS1204
N	CYS58
N	CYS60
N	CYS73
N	CYS76
N	ASP739
N	ASP741
N	ASP743
N	CYS1112
D	CYS60
N	CYS1194
N	CYS1201
N	CYS1204
D	CYS73
D	CYS76
D	ASP739
D	ASP741
D	ASP743
D	CYS1112
D	CYS1194

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)