2OKO

Z. mobilis tRNA guanine transglycosylase E235Q mutant apo-structure at pH 5.5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002099	biological_process	tRNA wobble guanine modification
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0008479	molecular_function	tRNA-guanosine(34) queuine transglycosylase activity
A	0008616	biological_process	queuosine biosynthetic process
A	0016757	molecular_function	glycosyltransferase activity
A	0046872	molecular_function	metal ion binding
A	0101030	biological_process	tRNA-guanine transglycosylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 500

Chain	Residue
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 700

Chain	Residue
A	ARG336
A	ALA20
A	ARG21
A	ARG82
A	SER308
A	HIS332

---

site_id	AC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL A 701

Chain	Residue
A	ALA19
A	THR27
A	GLY28
A	THR29
A	SER366
A	GOL712
A	HOH1072
A	HOH1108
A	HOH1122
A	HOH1148
A	HOH1167

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 702

Chain	Residue
A	PRO56
A	GLU57
A	GLY94
A	TRP95
A	ASP96
A	ARG97
A	HOH1179
A	HOH1209
A	HOH1272

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 703

Chain	Residue
A	ASN70
A	ASP102
A	GLN107
A	GLY261
A	ASP280
A	HOH1160

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 704

Chain	Residue
A	GLN117
A	ARG174
A	PRO252
A	ASP254
A	LYS255
A	HOH1051

---

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 705

Chain	Residue
A	ARG34
A	HIS145
A	GLY148
A	SER149
A	GLU191
A	GLN192
A	GOL708
A	HOH1022
A	HOH1079

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 706

Chain	Residue
A	GLU317
A	LYS360
A	PHE373
A	ARG380
A	HOH1025
A	HOH1201

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 707

Chain	Residue
A	MET172
A	GLU173
A	MET176
A	ARG177
A	ALA217
A	GOL715
A	HOH1172
A	HOH1181

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 708

Chain	Residue
A	ARG34
A	GLY87
A	GLY88
A	GLY148
A	GOL705
A	HOH1046
A	HOH1090

---

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 709

Chain	Residue
A	GLU81
A	GLU138
A	ARG139
A	GLU142
A	HOH1141
A	HOH1154
A	HOH1217
A	HOH1316

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 711

Chain	Residue
A	CYS158
A	GLY229
A	GLY230
A	LEU231
A	ALA232
A	HOH1202

---

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 712

Chain	Residue
A	ALA19
A	ASN304
A	ALA305
A	ARG306
A	SER366
A	GOL701
A	HOH1148

---

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL A 713

Chain	Residue
A	HOH1004
A	HOH1106

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 715

Chain	Residue
A	GOL707
A	HOH1188
A	MET176
A	ARG177
A	ALA217
A	ILE221

---

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 716

Chain	Residue
A	GLU22
A	LYS24
A	GLU273
A	PHE370
A	SER371

---

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 717

Chain	Residue
A	PRO249
A	MET250
A	LEU251
A	PRO252
A	ASP253
A	HOH1276

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	SER103

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	CYS281

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492

Chain	Residue	Details
A	SER103
A	GLU157
A	GLY204
A	LEU231

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936

Chain	Residue	Details
A	HIS319
A	ALA321
A	GLN324
A	ASN350

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1pud

Chain	Residue	Details
A	ASP102

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 881

Chain	Residue	Details
A	SER103	proton shuttle (general acid/base)
A	CYS281	covalent catalysis
A	HIS319	metal ligand
A	ALA321	metal ligand
A	GLN324	metal ligand
A	ASN350	metal ligand

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