Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2OJW

Crystal structure of human glutamine synthetase in complex with ADP and phosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001525	biological_process	angiogenesis
A	0003824	molecular_function	catalytic activity
A	0004356	molecular_function	glutamine synthetase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005783	cellular_component	endoplasmic reticulum
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006538	biological_process	glutamate catabolic process
A	0006542	biological_process	glutamine biosynthetic process
A	0008283	biological_process	cell population proliferation
A	0009267	biological_process	cellular response to starvation
A	0009749	biological_process	response to glucose
A	0010594	biological_process	regulation of endothelial cell migration
A	0016740	molecular_function	transferase activity
A	0016874	molecular_function	ligase activity
A	0018345	biological_process	protein palmitoylation
A	0019706	molecular_function	protein-cysteine S-palmitoyltransferase activity
A	0042254	biological_process	ribosome biogenesis
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0046872	molecular_function	metal ion binding
A	0070062	cellular_component	extracellular exosome
A	0097386	cellular_component	glial cell projection
A	1903670	biological_process	regulation of sprouting angiogenesis
A	1904749	biological_process	regulation of protein localization to nucleolus
B	0001525	biological_process	angiogenesis
B	0003824	molecular_function	catalytic activity
B	0004356	molecular_function	glutamine synthetase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005783	cellular_component	endoplasmic reticulum
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006538	biological_process	glutamate catabolic process
B	0006542	biological_process	glutamine biosynthetic process
B	0008283	biological_process	cell population proliferation
B	0009267	biological_process	cellular response to starvation
B	0009749	biological_process	response to glucose
B	0010594	biological_process	regulation of endothelial cell migration
B	0016740	molecular_function	transferase activity
B	0016874	molecular_function	ligase activity
B	0018345	biological_process	protein palmitoylation
B	0019706	molecular_function	protein-cysteine S-palmitoyltransferase activity
B	0042254	biological_process	ribosome biogenesis
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0046872	molecular_function	metal ion binding
B	0070062	cellular_component	extracellular exosome
B	0097386	cellular_component	glial cell projection
B	1903670	biological_process	regulation of sprouting angiogenesis
B	1904749	biological_process	regulation of protein localization to nucleolus
C	0001525	biological_process	angiogenesis
C	0003824	molecular_function	catalytic activity
C	0004356	molecular_function	glutamine synthetase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005783	cellular_component	endoplasmic reticulum
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006538	biological_process	glutamate catabolic process
C	0006542	biological_process	glutamine biosynthetic process
C	0008283	biological_process	cell population proliferation
C	0009267	biological_process	cellular response to starvation
C	0009749	biological_process	response to glucose
C	0010594	biological_process	regulation of endothelial cell migration
C	0016740	molecular_function	transferase activity
C	0016874	molecular_function	ligase activity
C	0018345	biological_process	protein palmitoylation
C	0019706	molecular_function	protein-cysteine S-palmitoyltransferase activity
C	0042254	biological_process	ribosome biogenesis
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0046872	molecular_function	metal ion binding
C	0070062	cellular_component	extracellular exosome
C	0097386	cellular_component	glial cell projection
C	1903670	biological_process	regulation of sprouting angiogenesis
C	1904749	biological_process	regulation of protein localization to nucleolus
D	0001525	biological_process	angiogenesis
D	0003824	molecular_function	catalytic activity
D	0004356	molecular_function	glutamine synthetase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005783	cellular_component	endoplasmic reticulum
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006538	biological_process	glutamate catabolic process
D	0006542	biological_process	glutamine biosynthetic process
D	0008283	biological_process	cell population proliferation
D	0009267	biological_process	cellular response to starvation
D	0009749	biological_process	response to glucose
D	0010594	biological_process	regulation of endothelial cell migration
D	0016740	molecular_function	transferase activity
D	0016874	molecular_function	ligase activity
D	0018345	biological_process	protein palmitoylation
D	0019706	molecular_function	protein-cysteine S-palmitoyltransferase activity
D	0042254	biological_process	ribosome biogenesis
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0046872	molecular_function	metal ion binding
D	0070062	cellular_component	extracellular exosome
D	0097386	cellular_component	glial cell projection
D	1903670	biological_process	regulation of sprouting angiogenesis
D	1904749	biological_process	regulation of protein localization to nucleolus
E	0001525	biological_process	angiogenesis
E	0003824	molecular_function	catalytic activity
E	0004356	molecular_function	glutamine synthetase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005783	cellular_component	endoplasmic reticulum
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006538	biological_process	glutamate catabolic process
E	0006542	biological_process	glutamine biosynthetic process
E	0008283	biological_process	cell population proliferation
E	0009267	biological_process	cellular response to starvation
E	0009749	biological_process	response to glucose
E	0010594	biological_process	regulation of endothelial cell migration
E	0016740	molecular_function	transferase activity
E	0016874	molecular_function	ligase activity
E	0018345	biological_process	protein palmitoylation
E	0019706	molecular_function	protein-cysteine S-palmitoyltransferase activity
E	0042254	biological_process	ribosome biogenesis
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0046872	molecular_function	metal ion binding
E	0070062	cellular_component	extracellular exosome
E	0097386	cellular_component	glial cell projection
E	1903670	biological_process	regulation of sprouting angiogenesis
E	1904749	biological_process	regulation of protein localization to nucleolus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 401

Chain	Residue
A	GLU136
A	GLU196
A	GLU203
A	MN404
A	PO4502
A	HOH1686

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 402

Chain	Residue
A	ARG340
A	ADP501
A	PO4502
A	GLU134
A	HIS253
A	GLU338

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 403

Chain	Residue
A	GLU134
A	GLU203
A	ADP501
A	PO4502
A	HOH1635

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 404

Chain	Residue
A	GLU196
A	MN401
A	PO4502
A	HOH1729
A	HOH1730
A	HOH1731

site_id	AC5
Number of Residues	16
Details	BINDING SITE FOR RESIDUE PO4 A 502

Chain	Residue
A	GLU134
A	GLU136
A	GLU196
A	GLU203
A	HIS253
A	ARG319
A	GLU338
A	ARG340
A	MN401
A	MN402
A	MN403
A	MN404
A	ADP501
A	HOH1686
A	HOH1730
A	HOH1786

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN B 401

Chain	Residue
B	GLU136
B	GLU196
B	GLU203
B	MN404
B	PO4502
B	HOH1747

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN B 402

Chain	Residue
B	GLU134
B	HIS253
B	GLU338
B	ARG340
B	ADP501
B	PO4502

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN B 403

Chain	Residue
B	GLU134
B	GLU203
B	ADP501
B	PO4502
B	HOH1616

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MN B 404

Chain	Residue
B	GLU196
B	MN401
B	PO4502
B	HOH1730
B	HOH1738
B	HOH1739
B	HOH1740

site_id	BC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PO4 B 502

Chain	Residue
B	GLU134
B	GLU136
B	GLU196
B	GLU203
B	HIS253
B	ARG319
B	GLU338
B	ARG340
B	MN401
B	MN402
B	MN403
B	MN404
B	ADP501
B	HOH1616
B	HOH1740

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 401

Chain	Residue
C	GLU136
C	GLU196
C	GLU203
C	MN404
C	PO4502
C	HOH1745

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 402

Chain	Residue
C	GLU134
C	HIS253
C	GLU338
C	ARG340
C	ADP501
C	PO4502

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN C 403

Chain	Residue
C	GLU134
C	GLU203
C	ADP501
C	PO4502
C	HOH1604

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 404

Chain	Residue
C	HOH1718
C	HOH1719
B	HOH1741
C	GLU196
C	MN401
C	PO4502

site_id	BC6
Number of Residues	17
Details	BINDING SITE FOR RESIDUE PO4 C 502

Chain	Residue
B	HOH1741
C	GLU134
C	GLU136
C	GLU196
C	GLU203
C	HIS253
C	ARG319
C	GLU338
C	ARG340
C	MN401
C	MN402
C	MN403
C	MN404
C	ADP501
C	HOH1707
C	HOH1718
C	HOH1745

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN D 401

Chain	Residue
D	GLU136
D	GLU196
D	GLU203
D	MN404
D	PO4502
D	HOH1741

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN D 402

Chain	Residue
D	GLU134
D	HIS253
D	GLU338
D	ARG340
D	ADP501
D	PO4502

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN D 403

Chain	Residue
D	GLU134
D	GLU203
D	ADP501
D	PO4502
D	HOH1615

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN D 404

Chain	Residue
D	GLU196
D	MN401
D	PO4502
D	HOH1609
D	HOH1739
D	HOH1740

site_id	CC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE PO4 D 502

Chain	Residue
D	GLU134
D	GLU136
D	GLU196
D	GLU203
D	HIS253
D	ARG319
D	GLU338
D	ARG340
D	MN401
D	MN402
D	MN403
D	MN404
D	ADP501
D	HOH1598
D	HOH1739
D	HOH1740
D	HOH1741

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN E 401

Chain	Residue
E	GLU136
E	GLU196
E	GLU203
E	MN404
E	PO4502
E	HOH1666

site_id	CC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN E 402

Chain	Residue
E	GLU134
E	HIS253
E	GLU338
E	ARG340
E	ADP501
E	PO4502

site_id	CC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN E 403

Chain	Residue
E	GLU134
E	GLU203
E	ADP501
E	PO4502
E	HOH1609

site_id	CC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MN E 404

Chain	Residue
E	GLU136
E	GLU196
E	MN401
E	PO4502
E	HOH1611
E	HOH1716
E	HOH1717

site_id	CC7
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PO4 E 502

Chain	Residue
E	GLU134
E	GLU136
E	GLU196
E	GLU203
E	HIS253
E	ARG319
E	GLU338
E	ARG340
E	MN401
E	MN402
E	MN403
E	MN404
E	ADP501
E	HOH1699
E	HOH1716

site_id	CC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL B 1401

Chain	Residue
B	THR44
C	THR193

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL C 1402

Chain	Residue
C	MET29
C	THR44
D	THR193

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 1403

Chain	Residue
A	THR193
E	THR44

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL D 1404

Chain	Residue
D	THR44
E	THR193
E	HOH1531

site_id	DC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 1405

Chain	Residue
A	THR44
B	THR193

site_id	DC4
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ADP A 501

Chain	Residue
A	TRP130
A	GLY132
A	GLU134
A	GLU203
A	GLN205
A	GLY207
A	PRO208
A	ASN255
A	SER257
A	ARG319
A	ARG324
A	TYR336
A	GLU338
A	MN402
A	MN403
A	PO4502
A	HOH1540
A	HOH1587
A	HOH1619
A	HOH1620
A	HOH1635
A	HOH1664
E	HOH1513

site_id	DC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ADP B 501

Chain	Residue
A	HOH1575
B	TRP130
B	GLY132
B	GLU134
B	GLU203
B	GLN205
B	GLY207
B	PRO208
B	ASN255
B	SER257
B	ARG319
B	ARG324
B	TYR336
B	GLU338
B	MN402
B	MN403
B	PO4502
B	HOH1560
B	HOH1570
B	HOH1589
B	HOH1616
B	HOH1693
B	HOH1753

site_id	DC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ADP C 501

Chain	Residue
C	TRP130
C	GLY132
C	GLU134
C	GLU203
C	GLN205
C	GLY207
C	PRO208
C	ASN255
C	SER257
C	ARG319
C	ARG324
C	TYR336
C	GLU338
C	MN402
C	MN403
C	PO4502
C	HOH1584
C	HOH1600
C	HOH1603
C	HOH1604
C	HOH1656
C	HOH1674
C	HOH1681

site_id	DC7
Number of Residues	24
Details	BINDING SITE FOR RESIDUE ADP D 501

Chain	Residue
D	TRP130
D	GLY132
D	GLU134
D	GLU203
D	GLN205
D	GLY207
D	PRO208
D	ASN255
D	SER257
D	ARG319
D	ARG324
D	TYR336
D	GLU338
D	MN402
D	MN403
D	PO4502
D	HOH1585
D	HOH1597
D	HOH1615
D	HOH1628
D	HOH1631
D	HOH1636
D	HOH1716
D	HOH1750

site_id	DC8
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ADP E 501

Chain	Residue
D	HOH1617
E	TRP130
E	GLY132
E	GLU134
E	GLU203
E	GLN205
E	GLY207
E	PRO208
E	ASN255
E	SER257
E	ARG262
E	ARG319
E	ARG324
E	TYR336
E	GLU338
E	MN402
E	MN403
E	PO4502
E	HOH1540
E	HOH1555
E	HOH1609
E	HOH1636
E	HOH1644

site_id	DC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 1501

Chain	Residue
A	LYS14
A	PRO88
A	PHE89
A	HOH1543
A	HOH1665
A	HOH1694

site_id	EC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 1502

Chain	Residue
A	TYR17
B	LYS14
B	PRO88
B	PHE89
B	HOH1548
B	HOH1674
B	HOH1748
B	HOH1756

site_id	EC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 1503

Chain	Residue
C	LYS14
C	PRO88
C	PHE89
C	HOH1557
C	HOH1734

site_id	EC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 1504

Chain	Residue
D	LYS14
D	PRO88
D	PHE89
D	HOH1601
D	HOH1752

site_id	EC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL E 1505

Chain	Residue
D	VAL16
E	LYS14
E	PRO88
E	PHE89
E	HOH1557
E	HOH1594
E	HOH1642

site_id	EC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL C 1506

Chain	Residue
C	ALA82
C	SER213
C	ASP216
C	HIS217
C	HOH1781

Functional Information from PROSITE/UniProt

site_id	PS00180
Number of Residues	18
Details	GLNA_1 Glutamine synthetase signature 1. FDGSStlqsegs.NSDmyL

Chain	Residue	Details
A	PHE62-LEU79

site_id	PS00181
Number of Residues	17
Details	GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPIpgnw.NGAGcHtnfS

Chain	Residue	Details
A	LYS241-SER257

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	30
Details	BINDING: BINDING => ECO:0007744\|PDB:2QC8

Chain	Residue	Details
A	GLN135
B	PHE204
B	THR254
B	ASP339
C	GLN135
C	TYR137
C	VAL197
C	PHE204
C	THR254
C	ASP339
D	GLN135
A	TYR137
D	TYR137
D	VAL197
D	PHE204
D	THR254
D	ASP339
E	GLN135
E	TYR137
E	VAL197
E	PHE204
E	THR254
A	VAL197
E	ASP339
A	PHE204
A	THR254
A	ASP339
B	GLN135
B	TYR137
B	VAL197

site_id	SWS_FT_FI2
Number of Residues	15
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P9WN39

Chain	Residue	Details
A	TRP247
D	TRP247
D	SER320
D	ARG341
E	TRP247
E	SER320
E	ARG341
A	SER320
A	ARG341
B	TRP247
B	SER320
B	ARG341
C	TRP247
C	SER320
C	ARG341

site_id	SWS_FT_FI3
Number of Residues	15
Details	BINDING: BINDING => ECO:0007744\|PDB:2OJW, ECO:0007744\|PDB:2QC8

Chain	Residue	Details
A	PHE256
D	PHE256
D	ILE325
D	PHE337
E	PHE256
E	ILE325
E	PHE337
A	ILE325
A	PHE337
B	PHE256
B	ILE325
B	PHE337
C	PHE256
C	ILE325
C	PHE337

site_id	SWS_FT_FI4
Number of Residues	5
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P15105

Chain	Residue	Details
A	ASN105
B	ASN105
C	ASN105
D	ASN105
E	ASN105

site_id	SWS_FT_FI5
Number of Residues	5
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	ALA344
B	ALA344
C	ALA344
D	ALA344
E	ALA344

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1hto

Chain	Residue	Details
A	ARG319
A	ASP63

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1hto

Chain	Residue	Details
B	ARG319
B	ASP63

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1hto

Chain	Residue	Details
C	ARG319
C	ASP63

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1hto

Chain	Residue	Details
D	ARG319
D	ASP63

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1hto

Chain	Residue	Details
E	ARG319
E	ASP63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)