2OIP

Crystal Structure of the S290G Active Site Mutant of TS-DHFR from Cryptosporidium hominis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004146	molecular_function	dihydrofolate reductase activity
A	0004799	molecular_function	thymidylate synthase activity
A	0006231	biological_process	dTMP biosynthetic process
A	0006730	biological_process	one-carbon metabolic process
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0046654	biological_process	tetrahydrofolate biosynthetic process
B	0004146	molecular_function	dihydrofolate reductase activity
B	0004799	molecular_function	thymidylate synthase activity
B	0006231	biological_process	dTMP biosynthetic process
B	0006730	biological_process	one-carbon metabolic process
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0046654	biological_process	tetrahydrofolate biosynthetic process
C	0004146	molecular_function	dihydrofolate reductase activity
C	0004799	molecular_function	thymidylate synthase activity
C	0006231	biological_process	dTMP biosynthetic process
C	0006730	biological_process	one-carbon metabolic process
C	0016741	molecular_function	transferase activity, transferring one-carbon groups
C	0046654	biological_process	tetrahydrofolate biosynthetic process
D	0004146	molecular_function	dihydrofolate reductase activity
D	0004799	molecular_function	thymidylate synthase activity
D	0006231	biological_process	dTMP biosynthetic process
D	0006730	biological_process	one-carbon metabolic process
D	0016741	molecular_function	transferase activity, transferring one-carbon groups
D	0046654	biological_process	tetrahydrofolate biosynthetic process
E	0004146	molecular_function	dihydrofolate reductase activity
E	0004799	molecular_function	thymidylate synthase activity
E	0006231	biological_process	dTMP biosynthetic process
E	0006730	biological_process	one-carbon metabolic process
E	0016741	molecular_function	transferase activity, transferring one-carbon groups
E	0046654	biological_process	tetrahydrofolate biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00075
Number of Residues	23
Details	DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGingqLPWsise.DlkfFskiT

Chain	Residue	Details
A	GLY18-THR40

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site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrhIltaWNpsalsqma.....LpPCHvlsQYyV

Chain	Residue	Details
A	ARG382-VAL410

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	6
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
A	ASP426
A	ASP462
A	GLU294
A	HIS464
A	SER424
A	CYS402

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site_id	CSA10
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
E	LEU25
E	ASP32

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site_id	CSA2
Number of Residues	6
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
D	ASP426
D	ASP462
D	GLU294
D	HIS464
D	SER424
D	CYS402

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site_id	CSA3
Number of Residues	6
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
C	ASP426
C	ASP462
C	GLU294
C	HIS464
C	SER424
C	CYS402

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site_id	CSA4
Number of Residues	6
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
E	ASP426
E	ASP462
E	GLU294
E	HIS464
E	SER424
E	CYS402

---

site_id	CSA5
Number of Residues	6
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
B	ASP426
B	ASP462
B	GLU294
B	HIS464
B	SER424
B	CYS402

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site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
A	LEU25
A	ASP32

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site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
B	LEU25
B	ASP32

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site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
C	LEU25
C	ASP32

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site_id	CSA9
Number of Residues	2
Details	Annotated By Reference To The Literature 1dhf

Chain	Residue	Details
D	LEU25
D	ASP32

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