Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2ODE

Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000166	molecular_function	nucleotide binding
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005886	cellular_component	plasma membrane
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0007194	biological_process	negative regulation of adenylate cyclase activity
A	0016020	cellular_component	membrane
A	0016239	biological_process	positive regulation of macroautophagy
A	0019001	molecular_function	guanyl nucleotide binding
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
A	0034451	cellular_component	centriolar satellite
A	0036064	cellular_component	ciliary basal body
A	0046039	biological_process	GTP metabolic process
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0070062	cellular_component	extracellular exosome
A	0097228	cellular_component	sperm principal piece
C	0000139	cellular_component	Golgi membrane
C	0000166	molecular_function	nucleotide binding
C	0001664	molecular_function	G protein-coupled receptor binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005794	cellular_component	Golgi apparatus
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
C	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
C	0007194	biological_process	negative regulation of adenylate cyclase activity
C	0016020	cellular_component	membrane
C	0016239	biological_process	positive regulation of macroautophagy
C	0019001	molecular_function	guanyl nucleotide binding
C	0019003	molecular_function	GDP binding
C	0030496	cellular_component	midbody
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0034451	cellular_component	centriolar satellite
C	0036064	cellular_component	ciliary basal body
C	0046039	biological_process	GTP metabolic process
C	0046872	molecular_function	metal ion binding
C	0051301	biological_process	cell division
C	0070062	cellular_component	extracellular exosome
C	0097228	cellular_component	sperm principal piece

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE ALF A 2001

Chain	Residue
A	GLY42
A	MG3001
A	HOH3229
A	HOH3231
A	HOH3236
A	GLU43
A	LYS46
A	ARG178
A	LYS180
A	THR181
A	GLY203
A	GLN204
A	GDP1001

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF C 2002

Chain	Residue
C	GLY42
C	GLU43
C	LYS46
C	ARG178
C	LYS180
C	THR181
C	VAL201
C	GLY203
C	GLN204
C	GDP1002
C	MG3002
C	HOH3187
C	HOH3190
C	HOH3194

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 3001

Chain	Residue
A	SER47
A	THR181
A	GDP1001
A	ALF2001
A	HOH3229
A	HOH3231

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 3002

Chain	Residue
C	SER47
C	THR181
C	GDP1002
C	ALF2002
C	HOH3187
C	HOH3190

site_id	AC5
Number of Residues	25
Details	BINDING SITE FOR RESIDUE GDP A 1001

Chain	Residue
A	GLU43
A	SER44
A	GLY45
A	LYS46
A	SER47
A	THR48
A	SER151
A	LEU175
A	ARG176
A	THR177
A	ARG178
A	ASN269
A	LYS270
A	ASP272
A	LEU273
A	CYS325
A	ALA326
A	THR327
A	ALF2001
A	MG3001
A	HOH3002
A	HOH3007
A	HOH3012
A	HOH3229
A	HOH3231

site_id	AC6
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDP C 1002

Chain	Residue
C	GLU43
C	SER44
C	GLY45
C	LYS46
C	SER47
C	THR48
C	SER151
C	LEU175
C	ARG176
C	THR177
C	ARG178
C	ASN269
C	LYS270
C	ASP272
C	LEU273
C	CYS325
C	ALA326
C	THR327
C	ALF2002
C	MG3002
C	HOH3007
C	HOH3008
C	HOH3018
C	HOH3187

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	18
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	14
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	10
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	44
Details	Binding site: {"evidences":[{"source":"PubMed","id":"37137991","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7X6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GVX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19478087","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2IHB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2ODE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V4Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"37137991","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2V4Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"ADP-ribosylarginine; by cholera toxin","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Deamidated glutamine; by Photorhabdus PAU_02230","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	230
Details	Domain: {"description":"RGS","evidences":[{"source":"PROSITE-ProRule","id":"PRU00171","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	THR181
A	GLN204
A	GLU43
A	ARG178

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	THR181
C	GLN204
C	GLU43
C	ARG178

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	GLN204

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	GLN204

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)