2O5X

Crystal structure of 1E9 LeuH47Trp/ArgH100Trp, an engineered Diels-Alderase Fab with nM steroid-binding affinity

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0002250	biological_process	adaptive immune response
L	0003823	molecular_function	antigen binding
L	0005576	cellular_component	extracellular region
L	0005886	cellular_component	plasma membrane
L	0006955	biological_process	immune response
L	0016064	biological_process	immunoglobulin mediated immune response
L	0019814	cellular_component	immunoglobulin complex
L	0050853	biological_process	B cell receptor signaling pathway
L	0070062	cellular_component	extracellular exosome
L	0071735	cellular_component	IgG immunoglobulin complex
L	0072562	cellular_component	blood microparticle

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 L 404

Chain	Residue
H	GLY55
H	GLU56
H	SER57
L	ARG211
L	HOH468
L	HOH515
L	HOH524

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 406

Chain	Residue
L	SER10
L	SER156
L	PRO8
L	LEU9

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 L 407

Chain	Residue
L	HIS93
L	PHE94

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 L 408

Chain	Residue
L	SER52
L	ASN53
L	ARG54
L	LYS188
L	HOH494

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 410

Chain	Residue
H	PRO123
L	SER121
L	ASP122
L	GLU123

---

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 H 401

Chain	Residue
H	PRO14
H	ASN84
H	SER112
H	SER113
H	HOH418
H	HOH505
L	ARG108
L	ALA111
L	HOH440
L	HOH454

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 402

Chain	Residue
H	SER25
H	GLY26
H	HOH531
H	HOH558

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 H 403

Chain	Residue
H	ARG66
H	LYS83
H	GLU85

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 H 405

Chain	Residue
H	GLU72
H	THR73
H	SER74

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 409

Chain	Residue
H	MET28
H	ASN31
H	ARG94
H	TYR102
H	HOH426

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 H 411

Chain	Residue
H	TYR27
H	MET28
H	MET28
H	TYR32
H	TYR32
H	HOH426

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE TRS H 301

Chain	Residue
H	ASN35
H	TRP50
H	TRP100
L	PHE89

---

Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YACEVTH

Chain	Residue	Details
L	TYR192-HIS198
H	TYR206-HIS212

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	Region: {"description":"Framework-1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	15
Details	Region: {"description":"Complementarity-determining-1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	Region: {"description":"Framework-2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Region: {"description":"Complementarity-determining-2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	31
Details	Region: {"description":"Framework-3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Region: {"description":"Complementarity-determining-3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	9
Details	Region: {"description":"Framework-4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	97
Details	Domain: {"description":"Ig-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00114","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	93
Details	Domain: {"description":"Ig-like 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00114","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	97
Details	Region: {"description":"CH1","evidences":[{"source":"PubMed","id":"15572068","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	20
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---