Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2NXH

Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
A	0009058	biological_process	biosynthetic process
A	0009103	biological_process	lipopolysaccharide biosynthetic process
A	0016740	molecular_function	transferase activity
A	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
A	0046364	biological_process	monosaccharide biosynthetic process
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
B	0009058	biological_process	biosynthetic process
B	0009103	biological_process	lipopolysaccharide biosynthetic process
B	0016740	molecular_function	transferase activity
B	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
B	0046364	biological_process	monosaccharide biosynthetic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
C	0009058	biological_process	biosynthetic process
C	0009103	biological_process	lipopolysaccharide biosynthetic process
C	0016740	molecular_function	transferase activity
C	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
C	0046364	biological_process	monosaccharide biosynthetic process
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
D	0009058	biological_process	biosynthetic process
D	0009103	biological_process	lipopolysaccharide biosynthetic process
D	0016740	molecular_function	transferase activity
D	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
D	0046364	biological_process	monosaccharide biosynthetic process
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
E	0009058	biological_process	biosynthetic process
E	0009103	biological_process	lipopolysaccharide biosynthetic process
E	0016740	molecular_function	transferase activity
E	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
E	0046364	biological_process	monosaccharide biosynthetic process
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
F	0009058	biological_process	biosynthetic process
F	0009103	biological_process	lipopolysaccharide biosynthetic process
F	0016740	molecular_function	transferase activity
F	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
F	0046364	biological_process	monosaccharide biosynthetic process
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
G	0009058	biological_process	biosynthetic process
G	0009103	biological_process	lipopolysaccharide biosynthetic process
G	0016740	molecular_function	transferase activity
G	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
G	0046364	biological_process	monosaccharide biosynthetic process
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
H	0009058	biological_process	biosynthetic process
H	0009103	biological_process	lipopolysaccharide biosynthetic process
H	0016740	molecular_function	transferase activity
H	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
H	0046364	biological_process	monosaccharide biosynthetic process
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
I	0009058	biological_process	biosynthetic process
I	0009103	biological_process	lipopolysaccharide biosynthetic process
I	0016740	molecular_function	transferase activity
I	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
I	0046364	biological_process	monosaccharide biosynthetic process
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
J	0009058	biological_process	biosynthetic process
J	0009103	biological_process	lipopolysaccharide biosynthetic process
J	0016740	molecular_function	transferase activity
J	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
J	0046364	biological_process	monosaccharide biosynthetic process
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
K	0009058	biological_process	biosynthetic process
K	0009103	biological_process	lipopolysaccharide biosynthetic process
K	0016740	molecular_function	transferase activity
K	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
K	0046364	biological_process	monosaccharide biosynthetic process
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0008676	molecular_function	3-deoxy-8-phosphooctulonate synthase activity
L	0009058	biological_process	biosynthetic process
L	0009103	biological_process	lipopolysaccharide biosynthetic process
L	0016740	molecular_function	transferase activity
L	0019294	biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
L	0046364	biological_process	monosaccharide biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 A 1280

Chain	Residue
A	ARG1049
A	SER1050
A	LYS1196
A	SER1197
A	HOH5079
A	HOH6212

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 B 1281

Chain	Residue
B	SER2197
B	HOH5152
B	HOH5484
B	HOH6483
B	ARG2049
B	SER2050
B	LYS2196

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 C 1282

Chain	Residue
C	ARG3049
C	SER3050
C	SER3197
C	HOH5889
C	HOH6214
D	ARG4106

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 D 1283

Chain	Residue
D	ARG4049
D	SER4050
D	LYS4196
D	SER4197
D	HOH5701
D	HOH6095

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 E 1284

Chain	Residue
E	ARG1049
E	SER1050
E	SER1197

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 F 1285

Chain	Residue
F	ARG2049
F	SER2050
F	LYS2196
F	SER2197
F	HOH6145

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 G 1286

Chain	Residue
G	ARG3049
G	SER3050
G	LYS3196
G	SER3197
G	HOH5692
G	HOH5916
G	HOH6516
G	HOH6529

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 H 1287

Chain	Residue
H	ARG4049
H	SER4050
H	HOH5429
H	HOH5879
H	HOH6044
H	HOH6463

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 I 1288

Chain	Residue
I	ARG1049
I	SER1050
I	LYS1196
I	SER1197
I	HOH5098
I	HOH5612

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 J 1289

Chain	Residue
J	ARG2049
J	SER2050
J	HOH5475
J	HOH5988

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 K 1290

Chain	Residue
K	ARG3049
K	SER3050
K	LYS3196
K	SER3197
K	HOH6104
K	HOH6207

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 L 1291

Chain	Residue
K	ARG3106
L	ARG4049
L	SER4050
L	SER4197
L	HOH6025
L	HOH6249

site_id	BC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP A 1268

Chain	Residue
A	LYS1041
A	SER1043
A	LYS1046
A	GLN1099
A	PRO1101
A	ALA1102
A	LYS1124
A	ARG1154
A	HIS1185
A	PHE1220
A	HOH5071
A	HOH5095
A	HOH5140
A	HOH5357

site_id	BC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP B 1269

Chain	Residue
B	LYS2041
B	SER2043
B	LYS2046
B	GLN2099
B	PRO2101
B	ALA2102
B	LYS2124
B	ARG2154
B	HIS2185
B	PHE2220
B	HOH5030
B	HOH5060
B	HOH5108
B	HOH5155

site_id	BC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PEP C 1270

Chain	Residue
C	LYS3046
C	GLN3099
C	PRO3101
C	ALA3102
C	LYS3124
C	ARG3154
C	HIS3185
C	PHE3220
C	HOH5543
C	HOH5587
C	HOH5607
C	LYS3041
C	SER3043

site_id	BC7
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PEP D 1271

Chain	Residue
D	ASN4011
D	LYS4041
D	SER4043
D	LYS4046
D	GLN4099
D	PRO4101
D	ALA4102
D	LYS4124
D	ARG4154
D	HIS4185
D	PHE4220
D	GLU4222
D	HOH5345
D	HOH5768
D	HOH5977

site_id	BC8
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PEP E 1272

Chain	Residue
E	ASN1011
E	LYS1041
E	SER1043
E	LYS1046
E	GLN1099
E	PRO1101
E	ALA1102
E	LYS1124
E	ARG1154
E	HIS1185
E	PHE1220
E	GLU1222
E	HOH5830

site_id	BC9
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP F 1273

Chain	Residue
F	LYS2041
F	SER2043
F	LYS2046
F	GLN2099
F	PRO2101
F	ALA2102
F	LYS2124
F	ARG2154
F	HIS2185
F	PHE2220
F	GLU2222
F	HOH5595
F	HOH5903
F	HOH6076

site_id	CC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP G 1274

Chain	Residue
G	LYS3041
G	SER3043
G	LYS3046
G	GLN3099
G	PRO3101
G	ALA3102
G	LYS3124
G	ARG3154
G	HIS3185
G	PHE3220
G	HOH5092
G	HOH5162
G	HOH5221
G	HOH5386

site_id	CC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP H 1275

Chain	Residue
H	LYS4041
H	SER4043
H	LYS4046
H	GLN4099
H	PRO4101
H	ALA4102
H	LYS4124
H	ARG4154
H	HIS4185
H	PHE4220
H	HOH5085
H	HOH5169
H	HOH5299
H	HOH5403

site_id	CC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP I 1276

Chain	Residue
I	LYS1041
I	SER1043
I	LYS1046
I	GLN1099
I	PRO1101
I	ALA1102
I	LYS1124
I	ARG1154
I	HIS1185
I	PHE1220
I	HOH5049
I	HOH5135
I	HOH5151
I	HOH5170

site_id	CC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PEP J 1277

Chain	Residue
J	LYS2041
J	SER2043
J	LYS2046
J	GLN2099
J	PRO2101
J	ALA2102
J	LYS2124
J	ARG2154
J	HIS2185
J	PHE2220
J	HOH5041
J	HOH5156
J	HOH5306
J	HOH5650

site_id	CC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PEP K 1278

Chain	Residue
K	ASN3011
K	LYS3041
K	SER3043
K	LYS3046
K	GLN3099
K	PRO3101
K	ALA3102
K	LYS3124
K	ARG3154
K	HIS3185
K	GLU3222
K	HOH5313
K	HOH5921

site_id	CC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PEP L 1279

Chain	Residue
L	LYS4041
L	SER4043
L	LYS4046
L	GLN4099
L	PRO4101
L	ALA4102
L	LYS4124
L	ARG4154
L	PHE4220
L	HOH5550
L	HOH5951
L	HOH6343
L	HOH6632

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)