2NXH
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.211, 198.628, 125.522 |
| Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
| Resolution | 30.180 - 2.110 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.123 |
| Number of reflections | 195362 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 91.8 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
