2L28

Solution structure of lactobacillus casei dihydrofolate reductase apo-form, 25 conformers

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004146	molecular_function	dihydrofolate reductase activity
A	0005829	cellular_component	cytosol
A	0006730	biological_process	one-carbon metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0031427	biological_process	response to methotrexate
A	0046452	biological_process	dihydrofolate metabolic process
A	0046654	biological_process	tetrahydrofolate biosynthetic process
A	0046655	biological_process	folic acid metabolic process
A	0046677	biological_process	response to antibiotic
A	0050661	molecular_function	NADP binding

Functional Information from PROSITE/UniProt

site_id	PS00075
Number of Residues	23
Details	DHFR_1 Dihydrofolate reductase (DHFR) domain signature. LIGkdghLPWhlpd.DlhyFraqT

Chain	Residue	Details
A	LEU12-THR34

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	BINDING: BINDING => ECO:0000305|PubMed:6815179

Chain	Residue	Details
A	LEU4
A	ASP26
A	ARG31
A	ARG57
A	THR116

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site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:6815179

Chain	Residue	Details
A	TRP5
A	ILE13
A	GLY42
A	LEU62
A	VAL79
A	ALA97

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site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: May be important for enzyme function

Chain	Residue	Details
A	TRP21

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	9
Details	M-CSA 112

Chain	Residue	Details
A	LEU4	steric role
A	LEU19	enhance reactivity
A	TRP21	activator, hydrogen bond donor
A	ASP26	hydrogen bond acceptor, modifies pKa
A	LEU27	steric locator
A	PHE30	steric locator
A	LEU54	steric locator
A	LEU94	steric role
A	THR116	electrostatic stabiliser, hydrogen bond donor

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