Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2J5T

Glutamate 5-kinase from Escherichia coli complexed with glutamate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003723	molecular_function	RNA binding
A	0004349	molecular_function	glutamate 5-kinase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006561	biological_process	obsolete proline biosynthetic process
A	0008652	biological_process	amino acid biosynthetic process
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0055129	biological_process	L-proline biosynthetic process
A	1901973	molecular_function	proline binding
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003723	molecular_function	RNA binding
B	0004349	molecular_function	glutamate 5-kinase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006561	biological_process	obsolete proline biosynthetic process
B	0008652	biological_process	amino acid biosynthetic process
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0055129	biological_process	L-proline biosynthetic process
B	1901973	molecular_function	proline binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003723	molecular_function	RNA binding
C	0004349	molecular_function	glutamate 5-kinase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006561	biological_process	obsolete proline biosynthetic process
C	0008652	biological_process	amino acid biosynthetic process
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0055129	biological_process	L-proline biosynthetic process
C	1901973	molecular_function	proline binding
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003723	molecular_function	RNA binding
D	0004349	molecular_function	glutamate 5-kinase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006561	biological_process	obsolete proline biosynthetic process
D	0008652	biological_process	amino acid biosynthetic process
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0055129	biological_process	L-proline biosynthetic process
D	1901973	molecular_function	proline binding
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0003723	molecular_function	RNA binding
E	0004349	molecular_function	glutamate 5-kinase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006561	biological_process	obsolete proline biosynthetic process
E	0008652	biological_process	amino acid biosynthetic process
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0055129	biological_process	L-proline biosynthetic process
E	1901973	molecular_function	proline binding
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0003723	molecular_function	RNA binding
F	0004349	molecular_function	glutamate 5-kinase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006561	biological_process	obsolete proline biosynthetic process
F	0008652	biological_process	amino acid biosynthetic process
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0055129	biological_process	L-proline biosynthetic process
F	1901973	molecular_function	proline binding
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0003723	molecular_function	RNA binding
G	0004349	molecular_function	glutamate 5-kinase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006561	biological_process	obsolete proline biosynthetic process
G	0008652	biological_process	amino acid biosynthetic process
G	0016301	molecular_function	kinase activity
G	0016740	molecular_function	transferase activity
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0055129	biological_process	L-proline biosynthetic process
G	1901973	molecular_function	proline binding
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0003723	molecular_function	RNA binding
H	0004349	molecular_function	glutamate 5-kinase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006561	biological_process	obsolete proline biosynthetic process
H	0008652	biological_process	amino acid biosynthetic process
H	0016301	molecular_function	kinase activity
H	0016740	molecular_function	transferase activity
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0055129	biological_process	L-proline biosynthetic process
H	1901973	molecular_function	proline binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A1372

Chain	Residue
A	GLY12
A	THR13
A	SER14
A	THR169

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A1375

Chain	Residue
A	ARG267

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A1376

Chain	Residue
A	ARG33
A	GLN37

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A1377

Chain	Residue
A	ARG265

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 B1370

Chain	Residue
B	GLY12
B	THR13
B	SER14
B	LEU168
B	THR169
B	ASP170
B	LYS217
B	LYS10

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL B1372

Chain	Residue
A	ARG82

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL B1373

Chain	Residue
B	ARG33
B	GLN37

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL B1374

Chain	Residue
B	ARG267

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C1370

Chain	Residue
C	SER14
C	THR169
C	LYS217

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL C1372

Chain	Residue
C	ARG33
C	GLN37

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG D1369

Chain	Residue
D	ASP204

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 D1370

Chain	Residue
D	GLY12
D	SER14
D	VAL15
D	LEU168
D	THR169
D	ASP170
D	LYS217

site_id	BC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL D1373

Chain	Residue
D	ARG33

site_id	BC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL D1374

Chain	Residue
D	ARG267

site_id	BC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG E1370

Chain	Residue
E	ASP204

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E1371

Chain	Residue
E	SER14
E	THR169
E	ASP170
E	LYS217

site_id	BC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL E1374

Chain	Residue
E	ARG33
G	HOH2001

site_id	BC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL E1375

Chain	Residue
E	ARG267

site_id	CC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL E1376

Chain	Residue
F	ALA67

site_id	CC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 F1370

Chain	Residue
F	LYS10
F	GLY12
F	THR13
F	SER14
F	THR169
F	ASP170
F	LYS217

site_id	CC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL F1373

Chain	Residue
F	ARG33

site_id	CC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL F1374

Chain	Residue
F	ARG267

site_id	CC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 G1370

Chain	Residue
G	GLY12
G	THR13
G	SER14
G	LEU168
G	THR169
G	LYS217

site_id	CC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL G1374

Chain	Residue
G	ARG309

site_id	CC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL G1375

Chain	Residue
G	ARG33
G	HOH2001

site_id	CC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 H1369

Chain	Residue
H	GLY12
H	THR13
H	SER14
H	THR169
H	ASP170
H	LYS217

site_id	CC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL H1370

Chain	Residue
H	ARG33
H	GLN37

site_id	DC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GLU A1373

Chain	Residue
A	ASP281
A	GLY283
A	ALA284
A	LEU296
A	LYS298
A	GLY299

site_id	DC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GLU A1374

Chain	Residue
A	SER50
A	GLY51
A	ALA52
A	ILE53
A	ASN134
A	ASP137
A	ASP148
A	ASN149
A	HOH2017

site_id	DC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GLU B1371

Chain	Residue
B	SER50
B	GLY51
B	ALA52
B	ILE53
B	ASN134
B	ASP137
B	ASN149

site_id	DC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GLU C1371

Chain	Residue
C	SER50
C	GLY51
C	ALA52
C	ASN134
C	ASP137
C	ASP148
C	ASN149

site_id	DC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GLU D1371

Chain	Residue
D	ASP281
D	GLY283
D	ALA284
D	ALA287
D	SER294

site_id	DC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GLU D1372

Chain	Residue
D	SER50
D	GLY51
D	ALA52
D	ILE53
D	ASN134
D	ASP137
D	ASP148
D	ASN149

site_id	DC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GLU E1372

Chain	Residue
E	ASP281
E	ALA284
E	LEU296
E	GLY299

site_id	DC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GLU E1373

Chain	Residue
E	SER50
E	ALA52
E	ILE53
E	ASN134
E	ASP137
E	ASP148
E	ASN149

site_id	DC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GLU F1371

Chain	Residue
F	ASP281
F	GLY283
F	ALA284
F	GLY299
F	HOH2012

site_id	EC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GLU F1372

Chain	Residue
F	SER50
F	GLY51
F	ALA52
F	ILE53
F	ASN134
F	ASP137
F	ASP148
F	ASN149

site_id	EC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GLU G1371

Chain	Residue
G	ASP281
G	GLY283
G	ALA284
G	LEU296
G	HOH2013

site_id	EC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GLU G1372

Chain	Residue
G	SER50
G	GLY51
G	ALA52
G	ILE53
G	ASN134
G	ASP137
G	ASP148
G	ASN149

Functional Information from PROSITE/UniProt

site_id	PS00902
Number of Residues	18
Details	GLUTAMATE_5_KINASE Glutamate 5-kinase signature. SglGtGGMsTKLqAAdvA

Chain	Residue	Details
A	SER207-ALA224

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	624
Details	Domain: {"description":"PUA","evidences":[{"source":"HAMAP-Rule","id":"MF_00456","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	28
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17321544","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2J5T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J5V","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)