2J5T
Glutamate 5-kinase from Escherichia coli complexed with glutamate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004349 | molecular_function | glutamate 5-kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006561 | biological_process | proline biosynthetic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0055129 | biological_process | L-proline biosynthetic process |
| A | 1901973 | molecular_function | proline binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004349 | molecular_function | glutamate 5-kinase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006561 | biological_process | proline biosynthetic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0016301 | molecular_function | kinase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0055129 | biological_process | L-proline biosynthetic process |
| B | 1901973 | molecular_function | proline binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003723 | molecular_function | RNA binding |
| C | 0004349 | molecular_function | glutamate 5-kinase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006561 | biological_process | proline biosynthetic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0016301 | molecular_function | kinase activity |
| C | 0016740 | molecular_function | transferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0042803 | molecular_function | protein homodimerization activity |
| C | 0055129 | biological_process | L-proline biosynthetic process |
| C | 1901973 | molecular_function | proline binding |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003723 | molecular_function | RNA binding |
| D | 0004349 | molecular_function | glutamate 5-kinase activity |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006561 | biological_process | proline biosynthetic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0016301 | molecular_function | kinase activity |
| D | 0016740 | molecular_function | transferase activity |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0042803 | molecular_function | protein homodimerization activity |
| D | 0055129 | biological_process | L-proline biosynthetic process |
| D | 1901973 | molecular_function | proline binding |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0003723 | molecular_function | RNA binding |
| E | 0004349 | molecular_function | glutamate 5-kinase activity |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006561 | biological_process | proline biosynthetic process |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0016301 | molecular_function | kinase activity |
| E | 0016740 | molecular_function | transferase activity |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0042803 | molecular_function | protein homodimerization activity |
| E | 0055129 | biological_process | L-proline biosynthetic process |
| E | 1901973 | molecular_function | proline binding |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0000287 | molecular_function | magnesium ion binding |
| F | 0003723 | molecular_function | RNA binding |
| F | 0004349 | molecular_function | glutamate 5-kinase activity |
| F | 0005524 | molecular_function | ATP binding |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006561 | biological_process | proline biosynthetic process |
| F | 0008652 | biological_process | amino acid biosynthetic process |
| F | 0016301 | molecular_function | kinase activity |
| F | 0016740 | molecular_function | transferase activity |
| F | 0042802 | molecular_function | identical protein binding |
| F | 0042803 | molecular_function | protein homodimerization activity |
| F | 0055129 | biological_process | L-proline biosynthetic process |
| F | 1901973 | molecular_function | proline binding |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0000287 | molecular_function | magnesium ion binding |
| G | 0003723 | molecular_function | RNA binding |
| G | 0004349 | molecular_function | glutamate 5-kinase activity |
| G | 0005524 | molecular_function | ATP binding |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0006561 | biological_process | proline biosynthetic process |
| G | 0008652 | biological_process | amino acid biosynthetic process |
| G | 0016301 | molecular_function | kinase activity |
| G | 0016740 | molecular_function | transferase activity |
| G | 0042802 | molecular_function | identical protein binding |
| G | 0042803 | molecular_function | protein homodimerization activity |
| G | 0055129 | biological_process | L-proline biosynthetic process |
| G | 1901973 | molecular_function | proline binding |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0000287 | molecular_function | magnesium ion binding |
| H | 0003723 | molecular_function | RNA binding |
| H | 0004349 | molecular_function | glutamate 5-kinase activity |
| H | 0005524 | molecular_function | ATP binding |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0006561 | biological_process | proline biosynthetic process |
| H | 0008652 | biological_process | amino acid biosynthetic process |
| H | 0016301 | molecular_function | kinase activity |
| H | 0016740 | molecular_function | transferase activity |
| H | 0042802 | molecular_function | identical protein binding |
| H | 0042803 | molecular_function | protein homodimerization activity |
| H | 0055129 | biological_process | L-proline biosynthetic process |
| H | 1901973 | molecular_function | proline binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A1372 |
| Chain | Residue |
| A | GLY12 |
| A | THR13 |
| A | SER14 |
| A | THR169 |
| site_id | AC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL A1375 |
| Chain | Residue |
| A | ARG267 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL A1376 |
| Chain | Residue |
| A | ARG33 |
| A | GLN37 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL A1377 |
| Chain | Residue |
| A | ARG265 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 B1370 |
| Chain | Residue |
| B | GLY12 |
| B | THR13 |
| B | SER14 |
| B | LEU168 |
| B | THR169 |
| B | ASP170 |
| B | LYS217 |
| B | LYS10 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL B1372 |
| Chain | Residue |
| A | ARG82 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL B1373 |
| Chain | Residue |
| B | ARG33 |
| B | GLN37 |
| site_id | AC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL B1374 |
| Chain | Residue |
| B | ARG267 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C1370 |
| Chain | Residue |
| C | SER14 |
| C | THR169 |
| C | LYS217 |
| site_id | BC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL C1372 |
| Chain | Residue |
| C | ARG33 |
| C | GLN37 |
| site_id | BC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE MG D1369 |
| Chain | Residue |
| D | ASP204 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 D1370 |
| Chain | Residue |
| D | GLY12 |
| D | SER14 |
| D | VAL15 |
| D | LEU168 |
| D | THR169 |
| D | ASP170 |
| D | LYS217 |
| site_id | BC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL D1373 |
| Chain | Residue |
| D | ARG33 |
| site_id | BC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL D1374 |
| Chain | Residue |
| D | ARG267 |
| site_id | BC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE MG E1370 |
| Chain | Residue |
| E | ASP204 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 E1371 |
| Chain | Residue |
| E | SER14 |
| E | THR169 |
| E | ASP170 |
| E | LYS217 |
| site_id | BC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL E1374 |
| Chain | Residue |
| E | ARG33 |
| G | HOH2001 |
| site_id | BC9 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL E1375 |
| Chain | Residue |
| E | ARG267 |
| site_id | CC1 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL E1376 |
| Chain | Residue |
| F | ALA67 |
| site_id | CC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 F1370 |
| Chain | Residue |
| F | LYS10 |
| F | GLY12 |
| F | THR13 |
| F | SER14 |
| F | THR169 |
| F | ASP170 |
| F | LYS217 |
| site_id | CC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL F1373 |
| Chain | Residue |
| F | ARG33 |
| site_id | CC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL F1374 |
| Chain | Residue |
| F | ARG267 |
| site_id | CC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 G1370 |
| Chain | Residue |
| G | GLY12 |
| G | THR13 |
| G | SER14 |
| G | LEU168 |
| G | THR169 |
| G | LYS217 |
| site_id | CC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL G1374 |
| Chain | Residue |
| G | ARG309 |
| site_id | CC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL G1375 |
| Chain | Residue |
| G | ARG33 |
| G | HOH2001 |
| site_id | CC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 H1369 |
| Chain | Residue |
| H | GLY12 |
| H | THR13 |
| H | SER14 |
| H | THR169 |
| H | ASP170 |
| H | LYS217 |
| site_id | CC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL H1370 |
| Chain | Residue |
| H | ARG33 |
| H | GLN37 |
| site_id | DC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GLU A1373 |
| Chain | Residue |
| A | ASP281 |
| A | GLY283 |
| A | ALA284 |
| A | LEU296 |
| A | LYS298 |
| A | GLY299 |
| site_id | DC2 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GLU A1374 |
| Chain | Residue |
| A | SER50 |
| A | GLY51 |
| A | ALA52 |
| A | ILE53 |
| A | ASN134 |
| A | ASP137 |
| A | ASP148 |
| A | ASN149 |
| A | HOH2017 |
| site_id | DC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GLU B1371 |
| Chain | Residue |
| B | SER50 |
| B | GLY51 |
| B | ALA52 |
| B | ILE53 |
| B | ASN134 |
| B | ASP137 |
| B | ASN149 |
| site_id | DC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GLU C1371 |
| Chain | Residue |
| C | SER50 |
| C | GLY51 |
| C | ALA52 |
| C | ASN134 |
| C | ASP137 |
| C | ASP148 |
| C | ASN149 |
| site_id | DC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GLU D1371 |
| Chain | Residue |
| D | ASP281 |
| D | GLY283 |
| D | ALA284 |
| D | ALA287 |
| D | SER294 |
| site_id | DC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GLU D1372 |
| Chain | Residue |
| D | SER50 |
| D | GLY51 |
| D | ALA52 |
| D | ILE53 |
| D | ASN134 |
| D | ASP137 |
| D | ASP148 |
| D | ASN149 |
| site_id | DC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GLU E1372 |
| Chain | Residue |
| E | ASP281 |
| E | ALA284 |
| E | LEU296 |
| E | GLY299 |
| site_id | DC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GLU E1373 |
| Chain | Residue |
| E | SER50 |
| E | ALA52 |
| E | ILE53 |
| E | ASN134 |
| E | ASP137 |
| E | ASP148 |
| E | ASN149 |
| site_id | DC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GLU F1371 |
| Chain | Residue |
| F | ASP281 |
| F | GLY283 |
| F | ALA284 |
| F | GLY299 |
| F | HOH2012 |
| site_id | EC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GLU F1372 |
| Chain | Residue |
| F | SER50 |
| F | GLY51 |
| F | ALA52 |
| F | ILE53 |
| F | ASN134 |
| F | ASP137 |
| F | ASP148 |
| F | ASN149 |
| site_id | EC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GLU G1371 |
| Chain | Residue |
| G | ASP281 |
| G | GLY283 |
| G | ALA284 |
| G | LEU296 |
| G | HOH2013 |
| site_id | EC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GLU G1372 |
| Chain | Residue |
| G | SER50 |
| G | GLY51 |
| G | ALA52 |
| G | ILE53 |
| G | ASN134 |
| G | ASP137 |
| G | ASP148 |
| G | ASN149 |
Functional Information from PROSITE/UniProt
| site_id | PS00902 |
| Number of Residues | 18 |
| Details | GLUTAMATE_5_KINASE Glutamate 5-kinase signature. SglGtGGMsTKLqAAdvA |
| Chain | Residue | Details |
| A | SER207-ALA224 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000305 |
| Chain | Residue | Details |
| A | LYS10 | |
| D | LYS10 | |
| D | THR169 | |
| D | THR211 | |
| E | LYS10 | |
| E | THR169 | |
| E | THR211 | |
| F | LYS10 | |
| F | THR169 | |
| F | THR211 | |
| G | LYS10 | |
| A | THR169 | |
| G | THR169 | |
| G | THR211 | |
| H | LYS10 | |
| H | THR169 | |
| H | THR211 | |
| A | THR211 | |
| B | LYS10 | |
| B | THR169 | |
| B | THR211 | |
| C | LYS10 | |
| C | THR169 | |
| C | THR211 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17321544, ECO:0007744|PDB:2J5T, ECO:0007744|PDB:2J5V |
| Chain | Residue | Details |
| A | SER50 | |
| D | SER50 | |
| D | ASP137 | |
| D | ASN149 | |
| E | SER50 | |
| E | ASP137 | |
| E | ASN149 | |
| F | SER50 | |
| F | ASP137 | |
| F | ASN149 | |
| G | SER50 | |
| A | ASP137 | |
| G | ASP137 | |
| G | ASN149 | |
| H | SER50 | |
| H | ASP137 | |
| H | ASN149 | |
| A | ASN149 | |
| B | SER50 | |
| B | ASP137 | |
| B | ASN149 | |
| C | SER50 | |
| C | ASP137 | |
| C | ASN149 |
