2J5T
Glutamate 5-kinase from Escherichia coli complexed with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.302, 124.108, 144.927 |
| Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIALLY-REFINED MODEL OF PBD ENTRY 2J5V |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 0.490 |
| Number of reflections | 74908 | |
| <I/σ(I)> | 9.7 | 2.9 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.45 M MGSO4, 20 MM CACL2, 0.1 M MES PH 6.5. |
