2IZZ
Crystal structure of human pyrroline-5-carboxylate reductase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006561 | biological_process | proline biosynthetic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0034599 | biological_process | cellular response to oxidative stress |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0051881 | biological_process | regulation of mitochondrial membrane potential |
| A | 0055129 | biological_process | L-proline biosynthetic process |
| A | 1903377 | biological_process | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| B | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006561 | biological_process | proline biosynthetic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0034599 | biological_process | cellular response to oxidative stress |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0051881 | biological_process | regulation of mitochondrial membrane potential |
| B | 0055129 | biological_process | L-proline biosynthetic process |
| B | 1903377 | biological_process | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| C | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005759 | cellular_component | mitochondrial matrix |
| C | 0006561 | biological_process | proline biosynthetic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0034599 | biological_process | cellular response to oxidative stress |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0051881 | biological_process | regulation of mitochondrial membrane potential |
| C | 0055129 | biological_process | L-proline biosynthetic process |
| C | 1903377 | biological_process | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| D | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005759 | cellular_component | mitochondrial matrix |
| D | 0006561 | biological_process | proline biosynthetic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0034599 | biological_process | cellular response to oxidative stress |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0051881 | biological_process | regulation of mitochondrial membrane potential |
| D | 0055129 | biological_process | L-proline biosynthetic process |
| D | 1903377 | biological_process | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| E | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005739 | cellular_component | mitochondrion |
| E | 0005759 | cellular_component | mitochondrial matrix |
| E | 0006561 | biological_process | proline biosynthetic process |
| E | 0008652 | biological_process | amino acid biosynthetic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0034599 | biological_process | cellular response to oxidative stress |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0051881 | biological_process | regulation of mitochondrial membrane potential |
| E | 0055129 | biological_process | L-proline biosynthetic process |
| E | 1903377 | biological_process | negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 33 |
| Details | BINDING SITE FOR RESIDUE NAD A 350 |
| Chain | Residue |
| A | ILE6 |
| A | LEU39 |
| A | ASN56 |
| A | ALA69 |
| A | VAL70 |
| A | LYS71 |
| A | PRO72 |
| A | ILE78 |
| A | CYS95 |
| A | ALA96 |
| A | ALA97 |
| A | GLY7 |
| A | MET121 |
| A | THR122 |
| A | THR124 |
| A | EDO1273 |
| A | HOH2026 |
| A | HOH2036 |
| A | HOH2146 |
| A | HOH2147 |
| A | HOH2148 |
| A | HOH2150 |
| A | ALA8 |
| A | HOH2151 |
| B | ASN230 |
| B | VAL231 |
| B | SER233 |
| A | GLY9 |
| A | GLN10 |
| A | LEU11 |
| A | SER33 |
| A | SER34 |
| A | PRO35 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO A1272 |
| Chain | Residue |
| A | VAL231 |
| A | THR238 |
| A | HOH2152 |
| B | GLY175 |
| B | NAD350 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO A1273 |
| Chain | Residue |
| A | GLY175 |
| A | NAD350 |
| A | HOH2153 |
| B | VAL231 |
| B | THR238 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE EDO A1274 |
| Chain | Residue |
| A | ASN230 |
| A | SER233 |
| A | HOH2113 |
| A | HOH2154 |
| B | LYS71 |
| B | NAD350 |
| site_id | AC5 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NAD B 350 |
| Chain | Residue |
| A | ASN230 |
| A | VAL231 |
| A | EDO1272 |
| A | EDO1274 |
| A | HOH2117 |
| B | GLY7 |
| B | ALA8 |
| B | GLY9 |
| B | GLN10 |
| B | LEU11 |
| B | SER34 |
| B | PRO35 |
| B | LEU39 |
| B | ASN56 |
| B | ALA69 |
| B | VAL70 |
| B | LYS71 |
| B | PRO72 |
| B | ILE78 |
| B | CYS95 |
| B | ALA96 |
| B | ALA97 |
| B | MET121 |
| B | THR122 |
| B | THR124 |
| B | HOH2038 |
| B | HOH2161 |
| B | HOH2162 |
| B | HOH2163 |
| B | HOH2164 |
| B | HOH2165 |
| B | HOH2166 |
| site_id | AC6 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE NAD C 350 |
| Chain | Residue |
| C | THR124 |
| C | EDO1277 |
| C | HOH2121 |
| C | HOH2122 |
| C | HOH2123 |
| C | HOH2124 |
| C | HOH2125 |
| C | HOH2126 |
| D | ASN230 |
| D | VAL231 |
| D | EDO1272 |
| C | ILE6 |
| C | GLY7 |
| C | ALA8 |
| C | GLY9 |
| C | GLN10 |
| C | LEU11 |
| C | SER34 |
| C | PRO35 |
| C | ASN56 |
| C | ALA69 |
| C | VAL70 |
| C | LYS71 |
| C | PRO72 |
| C | ILE78 |
| C | CYS95 |
| C | ALA96 |
| C | ALA97 |
| C | THR122 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO C1276 |
| Chain | Residue |
| C | VAL231 |
| C | SER233 |
| C | THR238 |
| D | NAD350 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO C1277 |
| Chain | Residue |
| C | ALA97 |
| C | THR171 |
| C | GLY175 |
| C | NAD350 |
| C | HOH2127 |
| D | VAL231 |
| D | THR238 |
| site_id | AC9 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE NAD D 350 |
| Chain | Residue |
| C | ASN230 |
| C | VAL231 |
| C | SER233 |
| C | EDO1276 |
| D | ALA8 |
| D | GLY9 |
| D | GLN10 |
| D | LEU11 |
| D | SER33 |
| D | SER34 |
| D | PRO35 |
| D | LEU39 |
| D | ASN56 |
| D | ALA69 |
| D | VAL70 |
| D | LYS71 |
| D | PRO72 |
| D | CYS95 |
| D | ALA96 |
| D | ALA97 |
| D | THR122 |
| D | THR124 |
| D | HOH2122 |
| D | HOH2123 |
| D | HOH2125 |
| D | HOH2126 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO D1272 |
| Chain | Residue |
| C | NAD350 |
| D | ASN230 |
| D | SER233 |
| D | HOH2127 |
| site_id | BC2 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE NAD E 350 |
| Chain | Residue |
| E | GLY7 |
| E | ALA8 |
| E | GLY9 |
| E | GLN10 |
| E | LEU11 |
| E | SER34 |
| E | PRO35 |
| E | LEU39 |
| E | ASN56 |
| E | ALA69 |
| E | VAL70 |
| E | LYS71 |
| E | PRO72 |
| E | CYS95 |
| E | ALA96 |
| E | ALA97 |
| E | THR122 |
| E | THR124 |
| E | VAL231 |
| E | EDO1273 |
| E | HOH2117 |
| E | HOH2118 |
| E | HOH2119 |
| E | HOH2120 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO E1273 |
| Chain | Residue |
| E | LYS71 |
| E | ASN230 |
| E | SER233 |
| E | NAD350 |
Functional Information from PROSITE/UniProt
| site_id | PS00521 |
| Number of Residues | 23 |
| Details | P5CR Delta 1-pyrroline-5-carboxylate reductase signature. Pgq.LkdnVSSpGGaTihALhvLE |
| Chain | Residue | Details |
| A | PRO224-GLU246 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 15 |
| Details | BINDING: BINDING => ECO:0000269|Ref.19 |
| Chain | Residue | Details |
| A | ILE6 | |
| D | ILE6 | |
| D | ALA69 | |
| D | CYS95 | |
| E | ILE6 | |
| E | ALA69 | |
| E | CYS95 | |
| A | ALA69 | |
| A | CYS95 | |
| B | ILE6 | |
| B | ALA69 | |
| B | CYS95 | |
| C | ILE6 | |
| C | ALA69 | |
| C | CYS95 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 30 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:28258219, ECO:0007744|PDB:5UAT, ECO:0007744|PDB:5UAV |
| Chain | Residue | Details |
| A | ALA8 | |
| B | ASP36 | |
| B | VAL70 | |
| B | ALA97 | |
| C | ALA8 | |
| C | GLN10 | |
| C | LEU11 | |
| C | ASP36 | |
| C | VAL70 | |
| C | ALA97 | |
| D | ALA8 | |
| A | GLN10 | |
| D | GLN10 | |
| D | LEU11 | |
| D | ASP36 | |
| D | VAL70 | |
| D | ALA97 | |
| E | ALA8 | |
| E | GLN10 | |
| E | LEU11 | |
| E | ASP36 | |
| E | VAL70 | |
| A | LEU11 | |
| E | ALA97 | |
| A | ASP36 | |
| A | VAL70 | |
| A | ALA97 | |
| B | ALA8 | |
| B | GLN10 | |
| B | LEU11 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:28258219, ECO:0007744|PDB:5UAT |
| Chain | Residue | Details |
| A | SER34 | |
| E | ASN230 | |
| A | ASN230 | |
| B | SER34 | |
| B | ASN230 | |
| C | SER34 | |
| C | ASN230 | |
| D | SER34 | |
| D | ASN230 | |
| E | SER34 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:28258219, ECO:0007744|PDB:5UAV |
| Chain | Residue | Details |
| A | ASN56 | |
| E | LYS71 | |
| A | LYS71 | |
| B | ASN56 | |
| B | LYS71 | |
| C | ASN56 | |
| C | LYS71 | |
| D | ASN56 | |
| D | LYS71 | |
| E | ASN56 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 15 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:28258219, ECO:0007744|PDB:5UAU |
| Chain | Residue | Details |
| A | GLU164 | |
| D | GLU164 | |
| D | ALA237 | |
| D | THR238 | |
| E | GLU164 | |
| E | ALA237 | |
| E | THR238 | |
| A | ALA237 | |
| A | THR238 | |
| B | GLU164 | |
| B | ALA237 | |
| B | THR238 | |
| C | GLU164 | |
| C | ALA237 | |
| C | THR238 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 5 |
| Details | MOD_RES: N-acetylserine => ECO:0000269|Ref.8, ECO:0000269|Ref.9, ECO:0007744|PubMed:19413330 |
| Chain | Residue | Details |
| A | SER2 | |
| B | SER2 | |
| C | SER2 | |
| D | SER2 | |
| E | SER2 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 5 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER278 | |
| B | SER278 | |
| C | SER278 | |
| D | SER278 | |
| E | SER278 |
