2IX1

RNase II D209N mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000175	molecular_function	3'-5'-RNA exonuclease activity
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006401	biological_process	RNA catabolic process
A	0006402	biological_process	mRNA catabolic process
A	0008408	molecular_function	3'-5' exonuclease activity
A	0008859	molecular_function	exoribonuclease II activity
A	0016070	biological_process	RNA metabolic process
A	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A1644

Chain	Residue
A	ASP201
A	ASP210
A	HOH2011
A	HOH2013
B	A12
B	A13

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Functional Information from PROSITE/UniProt

site_id	PS01175
Number of Residues	25
Details	RIBONUCLEASE_II Ribonuclease II family signature. HFGLglea.YAtWTSPIRKYgDminH

Chain	Residue	Details
A	HIS484-HIS508

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; by PatZ => ECO:0000269|PubMed:26847092

Chain	Residue	Details
A	LYS501

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 338

Chain	Residue	Details
A	ASP201	metal ligand
A	ASP207	metal ligand
A	ASN209	activator, electrostatic stabiliser, increase nucleophilicity
A	ASP210	metal ligand
A	TYR313	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG500	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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