2IX1
RNase II D209N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-14 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 86.316, 86.316, 279.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.300 - 2.740 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22500 |
Structure solution method | MIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.428 |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 2.850 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.080 | 0.640 |
Number of reflections | 30737 | |
<I/σ(I)> | 28.6 | 3.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | VAPOUR DIFFUSION IN SITTING DROPS WITH 1.5 MICRO-L OF PROTEIN SOLUTION, 20 MM TRIS-HCL PH 8, 150 MM NACL, 10% GLYCEROL AND 1 MM DTT, AND 1.5 MICRO-L OF WELL SOLUTION,2.4 M SODIUM MALONATE PH 6.0, AT 293 K |