2IS3

Crystal Structure of Escherichia coli RNase T

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000175	molecular_function	3'-5'-RNA exonuclease activity
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006259	biological_process	DNA metabolic process
A	0006396	biological_process	RNA processing
A	0006974	biological_process	DNA damage response
A	0008033	biological_process	tRNA processing
A	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
A	0008408	molecular_function	3'-5' exonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
A	0031125	biological_process	rRNA 3'-end processing
A	0034644	biological_process	cellular response to UV
A	0042780	biological_process	tRNA 3'-end processing
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043628	biological_process	regulatory ncRNA 3'-end processing
A	0045004	biological_process	DNA replication proofreading
A	0046872	molecular_function	metal ion binding
B	0000175	molecular_function	3'-5'-RNA exonuclease activity
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004518	molecular_function	nuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006259	biological_process	DNA metabolic process
B	0006396	biological_process	RNA processing
B	0006974	biological_process	DNA damage response
B	0008033	biological_process	tRNA processing
B	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
B	0008408	molecular_function	3'-5' exonuclease activity
B	0016787	molecular_function	hydrolase activity
B	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
B	0031125	biological_process	rRNA 3'-end processing
B	0034644	biological_process	cellular response to UV
B	0042780	biological_process	tRNA 3'-end processing
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0043628	biological_process	regulatory ncRNA 3'-end processing
B	0045004	biological_process	DNA replication proofreading
B	0046872	molecular_function	metal ion binding
C	0000175	molecular_function	3'-5'-RNA exonuclease activity
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004518	molecular_function	nuclease activity
C	0004527	molecular_function	exonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006259	biological_process	DNA metabolic process
C	0006396	biological_process	RNA processing
C	0006974	biological_process	DNA damage response
C	0008033	biological_process	tRNA processing
C	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
C	0008408	molecular_function	3'-5' exonuclease activity
C	0016787	molecular_function	hydrolase activity
C	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
C	0031125	biological_process	rRNA 3'-end processing
C	0034644	biological_process	cellular response to UV
C	0042780	biological_process	tRNA 3'-end processing
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043628	biological_process	regulatory ncRNA 3'-end processing
C	0045004	biological_process	DNA replication proofreading
C	0046872	molecular_function	metal ion binding
D	0000175	molecular_function	3'-5'-RNA exonuclease activity
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004518	molecular_function	nuclease activity
D	0004527	molecular_function	exonuclease activity
D	0004540	molecular_function	RNA nuclease activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006259	biological_process	DNA metabolic process
D	0006396	biological_process	RNA processing
D	0006974	biological_process	DNA damage response
D	0008033	biological_process	tRNA processing
D	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
D	0008408	molecular_function	3'-5' exonuclease activity
D	0016787	molecular_function	hydrolase activity
D	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
D	0031125	biological_process	rRNA 3'-end processing
D	0034644	biological_process	cellular response to UV
D	0042780	biological_process	tRNA 3'-end processing
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043628	biological_process	regulatory ncRNA 3'-end processing
D	0045004	biological_process	DNA replication proofreading
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 216

Chain	Residue
A	ARG15
A	HIS144
C	MSE173
C	ASP174
C	ARG189

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 216

Chain	Residue
A	SER137
A	LEU138
A	LYS139
A	ARG140

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 216

Chain	Residue
B	MSE173
B	ASP174
B	ARG189

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 217

Chain	Residue
B	LYS101
B	ARG104
B	ARG140

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 218

Chain	Residue
B	HIS97
B	ARG104
B	LEU138
B	LYS139
B	ARG140

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 217

Chain	Residue
C	ARG104
C	LEU138
C	LYS139
C	ARG140

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 216

Chain	Residue
D	LEU10
D	LYS45
D	MSE46
D	SER110

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 217

Chain	Residue
D	ARG15
D	HIS144

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 218

Chain	Residue
D	MSE173
D	ASP174
D	ARG189

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 217

Chain	Residue
A	MSE173
A	ASP174
A	ARG189

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 218

Chain	Residue
A	GLY9
A	LEU10
A	MSE46
A	SER110

---

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 219

Chain	Residue
B	GLY9
B	LEU10
B	LYS45
B	MSE46
B	SER110

---

site_id	BC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 218

Chain	Residue
C	LEU10
C	MSE46

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00157","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21317904","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22718982","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	18
Details	Site: {"description":"Important for substrate binding and specificity"}

Chain

Residue

Details

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