2IS3
Crystal Structure of Escherichia coli RNase T
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-26 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9786, 0.9788 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 213.140, 213.140, 149.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 3.100 |
| R-factor | 0.19735 |
| Rwork | 0.196 |
| R-free | 0.23143 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.092 | 0.696 |
| Number of reflections | 31325 | |
| <I/σ(I)> | 28.9 | 2.1 |
| Completeness [%] | 99.5 | 95.1 |
| Redundancy | 13 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.3 M ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
