2IS3
Crystal Structure of Escherichia coli RNase T
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-26 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9786, 0.9788 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 213.140, 213.140, 149.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.830 - 3.100 |
R-factor | 0.19735 |
Rwork | 0.196 |
R-free | 0.23143 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.369 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.092 | 0.696 |
Number of reflections | 31325 | |
<I/σ(I)> | 28.9 | 2.1 |
Completeness [%] | 99.5 | 95.1 |
Redundancy | 13 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.3 M ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |