2IOB
E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006749 | biological_process | glutathione metabolic process |
| A | 0008216 | biological_process | spermidine metabolic process |
| A | 0008884 | molecular_function | glutathionylspermidine amidase activity |
| A | 0008885 | molecular_function | glutathionylspermidine synthase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016874 | molecular_function | ligase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0006749 | biological_process | glutathione metabolic process |
| B | 0008216 | biological_process | spermidine metabolic process |
| B | 0008884 | molecular_function | glutathionylspermidine amidase activity |
| B | 0008885 | molecular_function | glutathionylspermidine synthase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016874 | molecular_function | ligase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 284 |
| Details | Domain: {"description":"Peptidase C51","evidences":[{"source":"PROSITE-ProRule","id":"PRU00048","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | Region: {"description":"Linker"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate","evidences":[{"source":"PubMed","id":"9398217","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 50 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Site: {"description":"Increases nucleophilicity of active site Cys; for amidase activity"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer; for synthetase activity"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Cysteine sulfenic acid (-SOH); transient","evidences":[{"source":"PubMed","id":"20530482","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
