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2IOB

E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein

Functional Information from GO Data
ChainGOidnamespacecontents
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005829cellular_componentcytosol
A0006749biological_processglutathione metabolic process
A0008216biological_processspermidine metabolic process
A0008884molecular_functionglutathionylspermidine amidase activity
A0008885molecular_functionglutathionylspermidine synthase activity
A0016787molecular_functionhydrolase activity
A0016874molecular_functionligase activity
A0046872molecular_functionmetal ion binding
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005829cellular_componentcytosol
B0006749biological_processglutathione metabolic process
B0008216biological_processspermidine metabolic process
B0008884molecular_functionglutathionylspermidine amidase activity
B0008885molecular_functionglutathionylspermidine synthase activity
B0016787molecular_functionhydrolase activity
B0016874molecular_functionligase activity
B0046872molecular_functionmetal ion binding
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate => ECO:0000269|PubMed:9398217
ChainResidueDetails
ACYS59
BCYS59

site_idSWS_FT_FI2
Number of Residues38
DetailsBINDING:
ChainResidueDetails
AGLN58
AGLU391
AGLU392
ATHR446
ALYS498
ALYS533
ACYS539
AGLN568
AGLN582
ALEU603
AASP610
AARG64
BGLN58
BARG64
BVAL78
BASN149
BARG316
BASP318
BGLU330
BASN332
BSER335
BGLU391
AVAL78
BGLU392
BTHR446
BLYS498
BLYS533
BCYS539
BGLN568
BGLN582
BLEU603
BASP610
AASN149
AARG316
AASP318
AGLU330
AASN332
ASER335

site_idSWS_FT_FI3
Number of Residues2
DetailsSITE: Increases nucleophilicity of active site Cys; for amidase activity
ChainResidueDetails
AHIS131
BHIS131

site_idSWS_FT_FI4
Number of Residues2
DetailsSITE: Transition state stabilizer; for synthetase activity
ChainResidueDetails
AARG316
BARG316

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: Cysteine sulfenic acid (-SOH); transient => ECO:0000269|PubMed:20530482
ChainResidueDetails
ACYS59
BCYS59

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PDB entries from 2024-07-24

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