2IOB
E. coli Bifunctional glutathionylspermidine synthetase/amidase Apo protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-11-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.267, 92.964, 108.296 |
Unit cell angles | 90.00, 109.37, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
Rwork | 0.175 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.929 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.076 |
Number of reflections | 70953 |
<I/σ(I)> | 18.8 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 12% PEG 3350, 0.6M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 12% PEG 3350, 0.6M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |