2IH2

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 5-methylpyrimidin-2(1H)-one at the target base partner position

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0006304	biological_process	DNA modification
A	0008168	molecular_function	methyltransferase activity
A	0009007	molecular_function	site-specific DNA-methyltransferase (adenine-specific) activity
A	0009307	biological_process	DNA restriction-modification system
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
D	0003676	molecular_function	nucleic acid binding
D	0003677	molecular_function	DNA binding
D	0006304	biological_process	DNA modification
D	0008168	molecular_function	methyltransferase activity
D	0009007	molecular_function	site-specific DNA-methyltransferase (adenine-specific) activity
D	0009307	biological_process	DNA restriction-modification system
D	0016740	molecular_function	transferase activity
D	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NEA A 2001

Chain	Residue
A	VAL21
A	ASN105
A	PRO107
A	PHE146
A	GOL3002
A	HOH3048
A	HOH3072
A	HOH3144
A	HOH3168
A	HOH3234
B	DA6
A	ALA47
A	GLU71
A	ILE72
A	ASP73
A	ALA76
A	ALA88
A	ASP89
A	PHE90

---

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE NEA D 2002

Chain	Residue
D	VAL21
D	ALA47
D	GLU71
D	ILE72
D	ASP73
D	ALA88
D	ASP89
D	PHE90
D	ASN105
D	PRO107
D	PHE146
D	GOL3003
D	HOH3066
D	HOH3164
D	HOH3173
D	HOH3205
E	DA6

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL C 3001

Chain	Residue
A	LYS139
A	ASP173
A	HOH3060
A	HOH3137
B	DG1
B	DT3
C	DA19
C	DC20
C	HOH3007
C	HOH3038

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 3002

Chain	Residue
A	TYR129
A	LEU142
A	NEA2001
A	HOH3255
A	HOH3381
A	HOH3420

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 3003

Chain	Residue
D	TYR129
D	LEU142
D	NEA2002
D	HOH3338
D	HOH3519

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL D 3004

Chain	Residue
A	LYS277
A	HOH3103
D	ARG58
D	THR63
D	TYR65
D	PHE67
D	TRP82
D	HOH3416

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 3005

Chain	Residue
D	LEU192
D	THR220
D	GLN221
D	GLU222
D	HOH3014
D	HOH3321

---

Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. ILGNPPY

Chain	Residue	Details
A	ILE102-TYR108

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	BINDING: BINDING => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	THR23
D	PRO107
A	GLU45
A	GLU71
A	ASP89
A	PRO107
D	THR23
D	GLU45
D	GLU71
D	ASP89

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Important for catalytic activity => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	ASN105
A	TYR108
D	ASN105
D	TYR108

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Important for catalytic activity; via amide nitrogen => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	PRO106
D	PRO106

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 2adm

Chain	Residue	Details
A	PRO106
A	ASN105
A	TYR108

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 2adm

Chain	Residue	Details
D	PRO106
D	ASN105
D	TYR108

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 46

Chain

Residue

Details

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site_id	MCSA2
Number of Residues	4
Details	M-CSA 46

Chain

Residue

Details

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