Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2IGN

Crystal structure of recombinant pyranose 2-oxidase H167A mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0016491	molecular_function	oxidoreductase activity
A	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
A	0042597	cellular_component	periplasmic space
A	0050233	molecular_function	pyranose oxidase activity
A	0050660	molecular_function	flavin adenine dinucleotide binding
B	0000166	molecular_function	nucleotide binding
B	0016491	molecular_function	oxidoreductase activity
B	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
B	0042597	cellular_component	periplasmic space
B	0050233	molecular_function	pyranose oxidase activity
B	0050660	molecular_function	flavin adenine dinucleotide binding
C	0000166	molecular_function	nucleotide binding
C	0016491	molecular_function	oxidoreductase activity
C	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
C	0042597	cellular_component	periplasmic space
C	0050233	molecular_function	pyranose oxidase activity
C	0050660	molecular_function	flavin adenine dinucleotide binding
D	0000166	molecular_function	nucleotide binding
D	0016491	molecular_function	oxidoreductase activity
D	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
D	0042597	cellular_component	periplasmic space
D	0050233	molecular_function	pyranose oxidase activity
D	0050660	molecular_function	flavin adenine dinucleotide binding
E	0000166	molecular_function	nucleotide binding
E	0016491	molecular_function	oxidoreductase activity
E	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
E	0042597	cellular_component	periplasmic space
E	0050233	molecular_function	pyranose oxidase activity
E	0050660	molecular_function	flavin adenine dinucleotide binding
F	0000166	molecular_function	nucleotide binding
F	0016491	molecular_function	oxidoreductase activity
F	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
F	0042597	cellular_component	periplasmic space
F	0050233	molecular_function	pyranose oxidase activity
F	0050660	molecular_function	flavin adenine dinucleotide binding
G	0000166	molecular_function	nucleotide binding
G	0016491	molecular_function	oxidoreductase activity
G	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
G	0042597	cellular_component	periplasmic space
G	0050233	molecular_function	pyranose oxidase activity
G	0050660	molecular_function	flavin adenine dinucleotide binding
H	0000166	molecular_function	nucleotide binding
H	0016491	molecular_function	oxidoreductase activity
H	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
H	0042597	cellular_component	periplasmic space
H	0050233	molecular_function	pyranose oxidase activity
H	0050660	molecular_function	flavin adenine dinucleotide binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	36
Details	BINDING SITE FOR RESIDUE FAD A 7001

Chain	Residue
A	VAL52
A	VAL160
A	GLY163
A	MET164
A	ALA167
A	TRP168
A	THR169
A	CYS170
A	ALA171
A	VAL281
A	CYS283
A	GLY53
A	THR319
A	ALA320
A	HIS324
A	LEU547
A	ASN593
A	THR595
A	HOH8006
A	HOH8007
A	HOH8012
A	HOH8018
A	GLY55
A	HOH8037
A	HOH8050
A	HOH8064
A	HOH8077
A	HOH8088
A	HOH8107
A	HOH8347
A	PRO56
A	ILE57
A	ASP76
A	ILE77
A	THR158
A	ARG159

site_id	AC2
Number of Residues	35
Details	BINDING SITE FOR RESIDUE FAD B 7002

Chain	Residue
B	VAL52
B	GLY53
B	GLY55
B	PRO56
B	ILE57
B	ASP76
B	ILE77
B	THR158
B	ARG159
B	VAL160
B	GLY163
B	MET164
B	TRP168
B	THR169
B	CYS170
B	ALA171
B	VAL281
B	CYS283
B	THR319
B	ALA320
B	HIS324
B	LEU547
B	ASN593
B	THR595
B	HOH8007
B	HOH8012
B	HOH8035
B	HOH8037
B	HOH8061
B	HOH8071
B	HOH8076
B	HOH8093
B	HOH8141
B	HOH8206
B	HOH8639

site_id	AC3
Number of Residues	35
Details	BINDING SITE FOR RESIDUE FAD D 7003

Chain	Residue
D	HOH8074
D	HOH8102
D	HOH8103
D	HOH8107
D	HOH8111
D	HOH8124
D	HOH8150
D	VAL52
D	GLY53
D	GLY55
D	PRO56
D	ILE57
D	ASP76
D	ILE77
D	THR158
D	ARG159
D	VAL160
D	GLY163
D	MET164
D	ALA167
D	TRP168
D	THR169
D	CYS170
D	ALA171
D	VAL281
D	CYS283
D	THR319
D	ALA320
D	HIS324
D	LEU547
D	ASN593
D	THR595
D	HOH8012
D	HOH8017
D	HOH8030

site_id	AC4
Number of Residues	36
Details	BINDING SITE FOR RESIDUE FAD C 7004

Chain	Residue
C	VAL52
C	GLY53
C	GLY55
C	PRO56
C	ILE57
C	ASP76
C	ILE77
C	THR158
C	ARG159
C	VAL160
C	GLY163
C	MET164
C	ALA167
C	TRP168
C	THR169
C	CYS170
C	ALA171
C	VAL281
C	CYS283
C	THR319
C	ALA320
C	HIS324
C	LEU547
C	ASN593
C	THR595
C	HOH8024
C	HOH8025
C	HOH8036
C	HOH8046
C	HOH8066
C	HOH8069
C	HOH8106
C	HOH8117
C	HOH8119
C	HOH8176
C	HOH8210

site_id	AC5
Number of Residues	35
Details	BINDING SITE FOR RESIDUE FAD E 7005

Chain	Residue
E	VAL52
E	GLY53
E	GLY55
E	PRO56
E	ILE57
E	ASP76
E	ILE77
E	THR158
E	ARG159
E	VAL160
E	GLY163
E	MET164
E	TRP168
E	THR169
E	CYS170
E	ALA171
E	VAL281
E	CYS283
E	THR319
E	ALA320
E	HIS324
E	LEU547
E	ASN593
E	THR595
E	HOH8012
E	HOH8018
E	HOH8034
E	HOH8037
E	HOH8072
E	HOH8105
E	HOH8106
E	HOH8122
E	HOH8160
E	HOH8211
E	HOH8269

site_id	AC6
Number of Residues	35
Details	BINDING SITE FOR RESIDUE FAD F 7006

Chain	Residue
F	VAL52
F	GLY53
F	GLY55
F	PRO56
F	ILE57
F	ASP76
F	ILE77
F	THR158
F	ARG159
F	VAL160
F	GLY163
F	MET164
F	TRP168
F	THR169
F	CYS170
F	ALA171
F	VAL281
F	CYS283
F	THR319
F	ALA320
F	HIS324
F	LEU547
F	ASN593
F	THR595
F	HOH8008
F	HOH8029
F	HOH8039
F	HOH8051
F	HOH8054
F	HOH8058
F	HOH8075
F	HOH8098
F	HOH8112
F	HOH8129
F	HOH8155

site_id	AC7
Number of Residues	37
Details	BINDING SITE FOR RESIDUE FAD H 7007

Chain	Residue
H	VAL52
H	GLY53
H	GLY55
H	PRO56
H	ILE57
H	ASP76
H	ILE77
H	ILE107
H	THR158
H	ARG159
H	VAL160
H	GLY163
H	MET164
H	ALA167
H	TRP168
H	THR169
H	CYS170
H	ALA171
H	VAL281
H	CYS283
H	THR319
H	ALA320
H	HIS324
H	LEU547
H	ASN593
H	THR595
H	HOH8006
H	HOH8020
H	HOH8027
H	HOH8038
H	HOH8041
H	HOH8065
H	HOH8104
H	HOH8171
H	HOH8173
H	HOH8176
H	HOH8198

site_id	AC8
Number of Residues	34
Details	BINDING SITE FOR RESIDUE FAD G 7008

Chain	Residue
G	GLY53
G	GLY55
G	PRO56
G	ILE57
G	ASP76
G	ILE77
G	THR158
G	ARG159
G	VAL160
G	GLY163
G	MET164
G	TRP168
G	THR169
G	CYS170
G	ALA171
G	VAL281
G	CYS283
G	THR319
G	ALA320
G	HIS324
G	LEU547
G	ASN593
G	THR595
G	HOH8009
G	HOH8012
G	HOH8028
G	HOH8030
G	HOH8066
G	HOH8111
G	HOH8123
G	HOH8140
G	HOH8151
G	HOH8187
G	HOH8228

site_id	AC9
Number of Residues	13
Details	BINDING SITE FOR RESIDUE MES F 8001

Chain	Residue
E	SER462
E	ILE463
E	ASP464
F	LEU121
F	VAL122
F	VAL123
F	TRP131
F	GLN132
F	ALA133
F	PHE137
F	ARG139
F	HOH8407
G	LEU149

site_id	BC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE MES A 8002

Chain	Residue
A	LEU121
A	VAL122
A	VAL123
A	TRP131
A	GLN132
A	ALA133
A	PHE137
A	ARG139
A	HOH8414
A	HOH8633
B	SER462
B	ILE463
B	ASP464
D	LEU149

site_id	BC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE MES B 8003

Chain	Residue
A	SER462
A	ILE463
A	ASP464
B	LEU121
B	VAL122
B	VAL123
B	TRP131
B	GLN132
B	ALA133
B	PHE137
B	ARG139
B	HOH8503
B	HOH8607
C	LEU149

site_id	BC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE MES H 8004

Chain	Residue
E	LEU149
G	SER462
G	ILE463
G	ASP464
H	LEU121
H	VAL123
H	TRP131
H	GLN132
H	ALA133
H	PHE137
H	ARG139
H	HOH8306

site_id	BC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE MES D 8005

Chain	Residue
A	LEU149
C	SER462
C	ILE463
C	ASP464
D	LEU121
D	VAL122
D	VAL123
D	TRP131
D	GLN132
D	ALA133
D	PHE137
D	ARG139
D	HOH8310

site_id	BC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE MES G 8006

Chain	Residue
F	LEU149
G	LEU121
G	VAL122
G	VAL123
G	TRP131
G	GLN132
G	ALA133
G	PHE137
G	ARG139
G	HOH8371
H	SER462
H	ILE463
H	ASP464

site_id	BC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE MES E 8007

Chain	Residue
E	LEU121
E	VAL122
E	VAL123
E	TRP131
E	GLN132
E	ALA133
E	PHE136
E	PHE137
E	ARG139
E	HOH8255
E	HOH8344
F	SER462
F	ILE463
F	ASP464
F	HOH8327
H	LEU149

site_id	BC7
Number of Residues	14
Details	BINDING SITE FOR RESIDUE MES C 8008

Chain	Residue
B	LEU149
C	LEU121
C	VAL122
C	VAL123
C	TRP131
C	GLN132
C	ALA133
C	PHE137
C	ARG139
C	HOH8251
D	SER462
D	ILE463
D	ASP464
D	HOH8439

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
A	ASN593
A	HIS548

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
B	ASN593
B	HIS548

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
D	ASN593
D	HIS548

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
C	ASN593
C	HIS548

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
E	ASN593
E	HIS548

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
F	ASN593
F	HIS548

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
H	ASN593
H	HIS548

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1kdg

Chain	Residue	Details
G	ASN593
G	HIS548

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)