2IGN

Crystal structure of recombinant pyranose 2-oxidase H167A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-5
Synchrotron site	MAX II
Beamline	I911-5
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9680
Spacegroup name	P 1 21 1
Unit cell lengths	168.066, 102.996, 168.560
Unit cell angles	90.00, 106.43, 90.00

Refinement procedure

Resolution	39.000 - 1.650
R-factor	0.17222
Rwork	0.172
R-free	0.20532
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.018
RMSD bond angle	1.778
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.000
High resolution limit [Å]	1.650
Number of reflections	660306
<I/σ(I)>	11.4
Completeness [%]	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	12-13% monomethyl ether PEG 2000, 0.1 M mes, 50 mM CaCl2, 25% glycerol , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K