2ID0

Escherichia coli RNase II

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006401	biological_process	RNA catabolic process
A	0006402	biological_process	mRNA catabolic process
A	0008408	molecular_function	3'-5' exonuclease activity
A	0008859	molecular_function	exoribonuclease II activity
A	0016070	biological_process	RNA metabolic process
A	0016078	biological_process	tRNA decay
A	0016787	molecular_function	hydrolase activity
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006401	biological_process	RNA catabolic process
B	0006402	biological_process	mRNA catabolic process
B	0008408	molecular_function	3'-5' exonuclease activity
B	0008859	molecular_function	exoribonuclease II activity
B	0016070	biological_process	RNA metabolic process
B	0016078	biological_process	tRNA decay
B	0016787	molecular_function	hydrolase activity
C	0003676	molecular_function	nucleic acid binding
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004527	molecular_function	exonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006401	biological_process	RNA catabolic process
C	0006402	biological_process	mRNA catabolic process
C	0008408	molecular_function	3'-5' exonuclease activity
C	0008859	molecular_function	exoribonuclease II activity
C	0016070	biological_process	RNA metabolic process
C	0016078	biological_process	tRNA decay
C	0016787	molecular_function	hydrolase activity
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004527	molecular_function	exonuclease activity
D	0004540	molecular_function	RNA nuclease activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006401	biological_process	RNA catabolic process
D	0006402	biological_process	mRNA catabolic process
D	0008408	molecular_function	3'-5' exonuclease activity
D	0008859	molecular_function	exoribonuclease II activity
D	0016070	biological_process	RNA metabolic process
D	0016078	biological_process	tRNA decay
D	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN A 1001

Chain	Residue
A	ASP201
A	ASP209
A	ASP210

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site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN B 1002

Chain	Residue
B	ASP201
B	ASP209
B	ASP210

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site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN C 1003

Chain	Residue
C	ASP201
C	ASP209
C	ASP210

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site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN D 1004

Chain	Residue
D	ASP201
D	ASP209
D	ASP210

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Functional Information from PROSITE/UniProt

site_id	PS01175
Number of Residues	25
Details	RIBONUCLEASE_II Ribonuclease II family signature. HFGLglea.YAtWTSPIRKYgDminH

Chain	Residue	Details
A	HIS484-HIS508

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine; by PatZ","evidences":[{"source":"PubMed","id":"26847092","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 338

Chain	Residue	Details
A	LYS221	metal ligand
A	ALA227	metal ligand
A	ALA233	activator, electrostatic stabiliser, increase nucleophilicity
A	TRP234	metal ligand
A	GLN345	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	MSE540	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 338

Chain	Residue	Details
B	LYS221	metal ligand
B	ALA227	metal ligand
B	ALA233	activator, electrostatic stabiliser, increase nucleophilicity
B	TRP234	metal ligand
B	GLN345	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	MSE540	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA3
Number of Residues	6
Details	M-CSA 338

Chain	Residue	Details
C	LYS221	metal ligand
C	ALA227	metal ligand
C	ALA233	activator, electrostatic stabiliser, increase nucleophilicity
C	TRP234	metal ligand
C	GLN345	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	MSE540	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA4
Number of Residues	6
Details	M-CSA 338

Chain	Residue	Details
D	LYS221	metal ligand
D	ALA227	metal ligand
D	ALA233	activator, electrostatic stabiliser, increase nucleophilicity
D	TRP234	metal ligand
D	GLN345	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	MSE540	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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