2ID0
Escherichia coli RNase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.760, 118.430, 122.380 |
| Unit cell angles | 107.80, 98.36, 91.40 |
Refinement procedure
| Resolution | 17.950 - 2.350 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.564 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.950 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.074 | 0.372 |
| Number of reflections | 117653 | |
| <I/σ(I)> | 23.5 | 2.8 |
| Completeness [%] | 92.7 | 69.6 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25.5% PEG 2000 MME, 0.36 M MgCl2, 0.5 mM MnCl2, 5 mM DTT, 2 mM 3',5'-ADP, 0.1 M Tris-Cl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
