Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2HOH

RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001411	cellular_component	hyphal tip
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0030428	cellular_component	cell septum
A	0046589	molecular_function	ribonuclease T1 activity
B	0001411	cellular_component	hyphal tip
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0030428	cellular_component	cell septum
B	0046589	molecular_function	ribonuclease T1 activity
C	0001411	cellular_component	hyphal tip
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0030428	cellular_component	cell septum
C	0046589	molecular_function	ribonuclease T1 activity
D	0001411	cellular_component	hyphal tip
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004521	molecular_function	RNA endonuclease activity
D	0004540	molecular_function	RNA nuclease activity
D	0016787	molecular_function	hydrolase activity
D	0016829	molecular_function	lyase activity
D	0030428	cellular_component	cell septum
D	0046589	molecular_function	ribonuclease T1 activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 300

Chain	Residue
C	GLY47
C	ASP49
C	HOH328
C	HOH330
D	TYR45
D	HOH328

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 301

Chain	Residue
C	TYR45
C	HOH334
C	HOH337
D	GLY47
D	ASP49

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA B 302

Chain	Residue
B	ASP3
B	THR104
B	HOH342
C	SER69

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 B 113

Chain	Residue
B	TYR38
B	GLU58
B	ARG77
B	HIS92
B	2GP304
D	ASN83

site_id	AC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE 2GP A 303

Chain	Residue
A	ASN36
A	TYR38
A	HIS40
A	LYS41
A	TYR42
A	ASN43
A	ASN44
A	TYR45
A	GLU46
A	GLU58
A	ASN98
A	PHE100
A	HOH312
A	HOH341
D	HOH359

site_id	AC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 2GP B 304

Chain	Residue
B	ASN36
B	TYR38
B	HIS40
B	LYS41
B	TYR42
B	ASN43
B	ASN44
B	TYR45
B	GLU46
B	GLU58
B	HIS92
B	ASN98
B	PHE100
B	PO4113
B	HOH344
D	ASN83

site_id	AC7
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 2GP C 305

Chain	Residue
C	ASN44
C	TYR45
C	GLU46
C	ASN99
C	PHE100
C	HOH310
C	HOH336
A	SER53
C	HIS40
C	LYS41
C	TYR42
C	ASN43

site_id	AC8
Number of Residues	21
Details	BINDING SITE FOR RESIDUE 2GP C 306

Chain	Residue
A	SER53
A	SER54
A	PRO55
A	ASN81
A	GLU82
A	ASN83
A	HOH314
C	ASN36
C	TYR38
C	HIS40
C	GLU58
C	SER72
C	PRO73
C	GLY74
C	ARG77
C	HIS92
C	ASN98
C	PHE100
C	HOH310
C	HOH336
C	HOH357

site_id	AC9
Number of Residues	13
Details	BINDING SITE FOR RESIDUE 2GP D 307

Chain	Residue
D	TYR38
D	HIS40
D	LYS41
D	TYR42
D	ASN43
D	ASN44
D	TYR45
D	GLU46
D	GLU58
D	ASN98
D	PHE100
D	HOH357
D	HOH360

site_id	BI1
Number of Residues	5
Details	BINDING SITE.

Chain	Residue
A	TYR42
A	ASN43
A	ASN44
A	TYR45
A	GLU46

site_id	BI2
Number of Residues	5
Details	BINDING SITE.

Chain	Residue
B	TYR42
B	ASN43
B	ASN44
B	TYR45
B	GLU46

site_id	BI3
Number of Residues	5
Details	BINDING SITE.

Chain	Residue
C	TYR42
C	ASN43
C	ASN44
C	TYR45
C	GLU46

site_id	BI4
Number of Residues	5
Details	BINDING SITE.

Chain	Residue
D	TYR42
D	ASN43
D	ASN44
D	TYR45
D	GLU46

site_id	CA1
Number of Residues	1
Details	SITE.

Chain	Residue
C	ASP49

site_id	CA2
Number of Residues	1
Details	SITE.

Chain	Residue
D	ASP49

site_id	CT1
Number of Residues	5
Details	ACTIVE SITE.

Chain	Residue
A	TYR38
A	HIS40
A	GLU58
A	ARG77
A	HIS92

site_id	CT2
Number of Residues	5
Details	ACTIVE SITE.

Chain	Residue
B	HIS92
B	TYR38
B	HIS40
B	GLU58
B	ARG77

site_id	CT3
Number of Residues	5
Details	ACTIVE SITE.

Chain	Residue
C	TYR38
C	HIS40
C	GLU58
C	ARG77
C	HIS92

site_id	CT4
Number of Residues	5
Details	ACTIVE SITE.

Chain	Residue
D	TYR38
D	HIS40
D	GLU58
D	ARG77
D	HIS92

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PubMed","id":"2844811","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"2844811","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton donor"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
A	GLU58
A	HIS40
A	HIS92

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
B	GLU58
B	HIS40
B	HIS92

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
C	GLU58
C	HIS40
C	HIS92

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1b2m

Chain	Residue	Details
D	GLU58
D	HIS40
D	HIS92

site_id	MCSA1
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
A	TYR38	electrostatic stabiliser
A	HIS40	proton shuttle (general acid/base)
A	GLU58	proton shuttle (general acid/base)
A	ARG77	electrostatic stabiliser
A	HIS92	proton shuttle (general acid/base)
A	PHE100	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
B	TYR38	electrostatic stabiliser
B	HIS40	proton shuttle (general acid/base)
B	GLU58	proton shuttle (general acid/base)
B	ARG77	electrostatic stabiliser
B	HIS92	proton shuttle (general acid/base)
B	PHE100	electrostatic stabiliser

site_id	MCSA3
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
C	TYR38	electrostatic stabiliser
C	HIS40	proton shuttle (general acid/base)
C	GLU58	proton shuttle (general acid/base)
C	ARG77	electrostatic stabiliser
C	HIS92	proton shuttle (general acid/base)
C	PHE100	electrostatic stabiliser

site_id	MCSA4
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
D	TYR38	electrostatic stabiliser
D	HIS40	proton shuttle (general acid/base)
D	GLU58	proton shuttle (general acid/base)
D	ARG77	electrostatic stabiliser
D	HIS92	proton shuttle (general acid/base)
D	PHE100	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)