2HOH
RIBONUCLEASE T1 (N9A MUTANT) COMPLEXED WITH 2'GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.370, 60.560, 101.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rga |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 28731 | |
| <I/σ(I)> | 13.34 | 4.38 |
| Completeness [%] | 98.0 | 93 |
| Redundancy | 7.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 25 MM NAAC PH 4.2 6.25 MM CACL2 45.0 % MPD |
