Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2HLN

L-asparaginase from Erwinia carotovora in complex with glutamic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004067	molecular_function	asparaginase activity
A	0006520	biological_process	amino acid metabolic process
A	0006528	biological_process	asparagine metabolic process
A	0016787	molecular_function	hydrolase activity
B	0004067	molecular_function	asparaginase activity
B	0006520	biological_process	amino acid metabolic process
B	0006528	biological_process	asparagine metabolic process
B	0016787	molecular_function	hydrolase activity
C	0004067	molecular_function	asparaginase activity
C	0006520	biological_process	amino acid metabolic process
C	0006528	biological_process	asparagine metabolic process
C	0016787	molecular_function	hydrolase activity
D	0004067	molecular_function	asparaginase activity
D	0006520	biological_process	amino acid metabolic process
D	0006528	biological_process	asparagine metabolic process
D	0016787	molecular_function	hydrolase activity
E	0004067	molecular_function	asparaginase activity
E	0006520	biological_process	amino acid metabolic process
E	0006528	biological_process	asparagine metabolic process
E	0016787	molecular_function	hydrolase activity
F	0004067	molecular_function	asparaginase activity
F	0006520	biological_process	amino acid metabolic process
F	0006528	biological_process	asparagine metabolic process
F	0016787	molecular_function	hydrolase activity
G	0004067	molecular_function	asparaginase activity
G	0006520	biological_process	amino acid metabolic process
G	0006528	biological_process	asparagine metabolic process
G	0016787	molecular_function	hydrolase activity
H	0004067	molecular_function	asparaginase activity
H	0006520	biological_process	amino acid metabolic process
H	0006528	biological_process	asparagine metabolic process
H	0016787	molecular_function	hydrolase activity
I	0004067	molecular_function	asparaginase activity
I	0006520	biological_process	amino acid metabolic process
I	0006528	biological_process	asparagine metabolic process
I	0016787	molecular_function	hydrolase activity
J	0004067	molecular_function	asparaginase activity
J	0006520	biological_process	amino acid metabolic process
J	0006528	biological_process	asparagine metabolic process
J	0016787	molecular_function	hydrolase activity
K	0004067	molecular_function	asparaginase activity
K	0006520	biological_process	amino acid metabolic process
K	0006528	biological_process	asparagine metabolic process
K	0016787	molecular_function	hydrolase activity
L	0004067	molecular_function	asparaginase activity
L	0006520	biological_process	amino acid metabolic process
L	0006528	biological_process	asparagine metabolic process
L	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GLU A 3350

Chain	Residue
A	GLY14
A	HOH3576
A	THR15
A	GLY61
A	SER62
A	GLU63
A	GLY94
A	THR95
A	ASP96
A	HOH3359

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GLU J 3351

Chain	Residue
J	GLY14
J	THR15
J	GLY61
J	SER62
J	GLU63
J	GLY94
J	THR95
J	ASP96
J	ALA120
J	HOH3374

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GLU E 3352

Chain	Residue
E	THR15
E	GLY61
E	SER62
E	GLU63
E	GLY94
E	THR95
E	ASP96
E	ALA120
E	HOH3406
E	HOH3448

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GLU F 3353

Chain	Residue
F	GLY14
F	THR15
F	GLY61
F	SER62
F	GLU63
F	GLY94
F	THR95
F	ASP96
F	ALA120
F	HOH3366

site_id	AC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GLU C 3354

Chain	Residue
C	GLY14
C	THR15
C	GLY61
C	SER62
C	GLU63
C	GLY94
C	THR95
C	ASP96
C	ALA120
C	HOH3432
C	HOH3483

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU D 3355

Chain	Residue
D	GLY14
D	THR15
D	GLY61
D	SER62
D	GLU63
D	GLY94
D	THR95
D	ASP96
D	ALA120
D	HOH3381
D	HOH3388
D	HOH3400

site_id	AC7
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU G 3356

Chain	Residue
G	GLY14
G	THR15
G	GLY61
G	SER62
G	GLU63
G	GLY94
G	THR95
G	ASP96
G	ALA120
G	HOH3403
G	HOH3459
G	HOH3489

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GLU H 3357

Chain	Residue
H	GLY14
H	THR15
H	GLY61
H	SER62
H	GLU63
H	GLY94
H	THR95
H	ASP96
H	ALA120

site_id	AC9
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GLU I 3358

Chain	Residue
I	GLY14
I	THR15
I	GLY61
I	SER62
I	GLU63
I	GLY94
I	THR95
I	ASP96
I	ALA120
I	HOH3420
I	HOH3453

site_id	BC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GLU B 3359

Chain	Residue
B	GLU63
B	GLY94
B	THR95
B	ASP96
B	ALA120
B	HOH3362
B	HOH3399
B	HOH3464
B	GLY14
B	THR15
B	GLY61
B	SER62

site_id	BC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GLU K 3360

Chain	Residue
K	GLY14
K	THR15
K	GLY61
K	SER62
K	GLU63
K	GLY94
K	THR95
K	ASP96
K	ALA120

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GLU L 3361

Chain	Residue
L	THR15
L	GLY61
L	SER62
L	GLU63
L	GLY94
L	THR95
L	ASP96
L	ALA120
L	HOH3421

site_id	BC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PEG K 2001

Chain	Residue
I	ILE222
I	TYR224
I	HOH3415
K	ILE222
K	ILE223
K	TYR224
K	TYR226
K	PRO230
K	MET233

site_id	BC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PEG A 2002

Chain	Residue
A	VAL202
A	ALA326
A	HOH3380
A	HOH3462
A	HOH3511
G	GLU231
G	ALA261
G	LYS265
G	PEG2006

site_id	BC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PEG B 2003

Chain	Residue
B	ILE222
B	ILE223
B	TYR224
B	TYR226
B	PRO230
B	MET233
F	ILE222
F	ILE223
F	TYR224

site_id	BC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PEG C 2004

Chain	Residue
A	TYR323
A	HOH3446
C	PRO218
C	ALA219
C	HOH3546
C	HOH3548
G	ASP228

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG K 2005

Chain	Residue
K	PRO218
K	ALA219
K	HOH3506

site_id	BC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PEG G 2006

Chain	Residue
A	ASP200
A	PEG2002
F	ASN146
F	ARG198
G	ALA261
G	ARG264
G	LYS265

site_id	CC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG K 2007

Chain	Residue
K	ASP200
K	LYS201
K	VAL202
K	HIS203
K	ARG206

site_id	CC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG K 2008

Chain	Residue
K	THR205
K	ARG206
K	HOH3499

Functional Information from PROSITE/UniProt

site_id	PS00144
Number of Residues	9
Details	ASN_GLN_ASE_1 Asparaginase / glutaminase active site signature 1. IlATGGTIA

Chain	Residue	Details
A	ILE9-ALA17

site_id	PS00917
Number of Residues	11
Details	ASN_GLN_ASE_2 Asparaginase / glutaminase active site signature 2. GvVitHGTDTL

Chain	Residue	Details
A	GLY88-LEU98

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	THR15
A	THR95
A	ASP96
A	LYS168

site_id	CSA10
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
J	THR15
J	THR95
J	ASP96
J	LYS168

site_id	CSA11
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
K	THR15
K	THR95
K	ASP96
K	LYS168

site_id	CSA12
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
L	THR15
L	THR95
L	ASP96
L	LYS168

site_id	CSA13
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	GLN290
E	THR15
E	THR95

site_id	CSA14
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
F	THR15
F	THR95
B	GLN290

site_id	CSA15
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	THR15
A	THR95
E	GLN290

site_id	CSA16
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
F	GLN290
B	THR15
B	THR95

site_id	CSA17
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
C	GLN290
G	THR15
G	THR95

site_id	CSA18
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
H	THR15
H	THR95
D	GLN290

site_id	CSA19
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
C	THR15
C	THR95
G	GLN290

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
B	THR15
B	THR95
B	ASP96
B	LYS168

site_id	CSA20
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
H	GLN290
D	THR15
D	THR95

site_id	CSA21
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
I	GLN290
K	THR15
K	THR95

site_id	CSA22
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
J	GLN290
L	THR15
L	THR95

site_id	CSA23
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
K	GLN290
I	THR15
I	THR95

site_id	CSA24
Number of Residues	3
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
J	THR15
J	THR95
L	GLN290

site_id	CSA25
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
A	GLY289

site_id	CSA26
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
B	GLY289

site_id	CSA27
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
E	GLY289

site_id	CSA28
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
F	GLY289

site_id	CSA29
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
C	GLY289

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
E	THR15
E	THR95
E	ASP96
E	LYS168

site_id	CSA30
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
D	GLY289

site_id	CSA31
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
G	GLY289

site_id	CSA32
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
H	GLY289

site_id	CSA33
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
I	GLY289

site_id	CSA34
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
J	GLY289

site_id	CSA35
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
K	GLY289

site_id	CSA36
Number of Residues	1
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
L	GLY289

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
F	THR15
F	THR95
F	ASP96
F	LYS168

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
C	THR15
C	THR95
C	ASP96
C	LYS168

site_id	CSA6
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
D	THR15
D	THR95
D	ASP96
D	LYS168

site_id	CSA7
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
G	THR15
G	THR95
G	ASP96
G	LYS168

site_id	CSA8
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
H	THR15
H	THR95
H	ASP96
H	LYS168

site_id	CSA9
Number of Residues	4
Details	Annotated By Reference To The Literature 3eca

Chain	Residue	Details
I	THR15
I	THR95
I	ASP96
I	LYS168

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)