2HLN
L-asparaginase from Erwinia carotovora in complex with glutamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.107, 123.675, 197.454 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.200 |
| R-factor | 0.22198 |
| Rwork | 0.220 |
| R-free | 0.26744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zcf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.423 |
| Number of reflections | 180862 | |
| <I/σ(I)> | 17.2 | 2.6 |
| Completeness [%] | 91.8 | 91 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1% sodium cacodilate, 8% PEG MME 5000, 0.06% octylglucoside, 0.08M glutamic acid, 0.02% sodium azide, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
