2GGK
The mutant A302C of Agrobacterium radiobacter N-carbamoyl-D-amino-acid amidohydrolase
Replaces: 2FKVReplaces: 2BA5Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| A | 0047417 | molecular_function | N-carbamoyl-D-amino acid hydrolase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| B | 0047417 | molecular_function | N-carbamoyl-D-amino acid hydrolase activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| C | 0047417 | molecular_function | N-carbamoyl-D-amino acid hydrolase activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| D | 0047417 | molecular_function | N-carbamoyl-D-amino acid hydrolase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1084 |
| Details | Domain: {"description":"CN hydrolase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"11237598","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| A | CYS172 | |
| A | GLU47 | |
| A | LYS127 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| B | CYS172 | |
| B | GLU47 | |
| B | LYS127 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| C | CYS172 | |
| C | GLU47 | |
| C | LYS127 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| D | CYS172 | |
| D | GLU47 | |
| D | LYS127 |
| site_id | CSA5 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| A | CYS172 | |
| A | GLU47 | |
| A | ASN197 | |
| A | LYS127 | |
| A | ASN110 | |
| A | GLU146 |
| site_id | CSA6 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| B | CYS172 | |
| B | GLU47 | |
| B | ASN197 | |
| B | LYS127 | |
| B | ASN110 | |
| B | GLU146 |
| site_id | CSA7 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| C | CYS172 | |
| C | GLU47 | |
| C | ASN197 | |
| C | LYS127 | |
| C | ASN110 | |
| C | GLU146 |
| site_id | CSA8 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1uf7 |
| Chain | Residue | Details |
| D | CYS172 | |
| D | GLU47 | |
| D | ASN197 | |
| D | LYS127 | |
| D | ASN110 | |
| D | GLU146 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 671 |
| Chain | Residue | Details |
| A | GLU47 | proton acceptor, proton donor |
| A | ASN110 | electrostatic stabiliser |
| A | LYS127 | electrostatic stabiliser |
| A | GLU146 | electrostatic stabiliser |
| A | CYS172 | nucleofuge, nucleophile, proton acceptor, proton donor |
| A | ASN197 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 671 |
| Chain | Residue | Details |
| B | GLU47 | proton acceptor, proton donor |
| B | ASN110 | electrostatic stabiliser |
| B | LYS127 | electrostatic stabiliser |
| B | GLU146 | electrostatic stabiliser |
| B | CYS172 | nucleofuge, nucleophile, proton acceptor, proton donor |
| B | ASN197 | electrostatic stabiliser |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 671 |
| Chain | Residue | Details |
| C | GLU47 | proton acceptor, proton donor |
| C | ASN110 | electrostatic stabiliser |
| C | LYS127 | electrostatic stabiliser |
| C | GLU146 | electrostatic stabiliser |
| C | CYS172 | nucleofuge, nucleophile, proton acceptor, proton donor |
| C | ASN197 | electrostatic stabiliser |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 671 |
| Chain | Residue | Details |
| D | GLU47 | proton acceptor, proton donor |
| D | ASN110 | electrostatic stabiliser |
| D | LYS127 | electrostatic stabiliser |
| D | GLU146 | electrostatic stabiliser |
| D | CYS172 | nucleofuge, nucleophile, proton acceptor, proton donor |
| D | ASN197 | electrostatic stabiliser |
