2GGK
The mutant A302C of Agrobacterium radiobacter N-carbamoyl-D-amino-acid amidohydrolase
Replaces: 2FKVReplaces: 2BA5Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.091, 67.819, 138.037 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.18021 |
| Rwork | 0.178 |
| R-free | 0.21963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fo6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 56903 | |
| Completeness [%] | 95.6 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | lithium sulfate, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
