Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2GCG

Ternary Crystal Structure of Human Glyoxylate Reductase/Hydroxypyruvate Reductase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005782	cellular_component	peroxisomal matrix
A	0005829	cellular_component	cytosol
A	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
A	0019752	biological_process	carboxylic acid metabolic process
A	0030267	molecular_function	glyoxylate reductase (NADPH) activity
A	0031406	molecular_function	carboxylic acid binding
A	0042803	molecular_function	protein homodimerization activity
A	0043648	biological_process	dicarboxylic acid metabolic process
A	0046487	biological_process	glyoxylate metabolic process
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
A	0070062	cellular_component	extracellular exosome
A	0070402	molecular_function	NADPH binding
A	0120509	molecular_function	hydroxypyruvate reductase (NADPH) activity
A	1902494	cellular_component	catalytic complex
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005782	cellular_component	peroxisomal matrix
B	0005829	cellular_component	cytosol
B	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
B	0019752	biological_process	carboxylic acid metabolic process
B	0030267	molecular_function	glyoxylate reductase (NADPH) activity
B	0031406	molecular_function	carboxylic acid binding
B	0042803	molecular_function	protein homodimerization activity
B	0043648	biological_process	dicarboxylic acid metabolic process
B	0046487	biological_process	glyoxylate metabolic process
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
B	0070062	cellular_component	extracellular exosome
B	0070402	molecular_function	NADPH binding
B	0120509	molecular_function	hydroxypyruvate reductase (NADPH) activity
B	1902494	cellular_component	catalytic complex
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005782	cellular_component	peroxisomal matrix
C	0005829	cellular_component	cytosol
C	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
C	0019752	biological_process	carboxylic acid metabolic process
C	0030267	molecular_function	glyoxylate reductase (NADPH) activity
C	0031406	molecular_function	carboxylic acid binding
C	0042803	molecular_function	protein homodimerization activity
C	0043648	biological_process	dicarboxylic acid metabolic process
C	0046487	biological_process	glyoxylate metabolic process
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
C	0070062	cellular_component	extracellular exosome
C	0070402	molecular_function	NADPH binding
C	0120509	molecular_function	hydroxypyruvate reductase (NADPH) activity
C	1902494	cellular_component	catalytic complex
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005782	cellular_component	peroxisomal matrix
D	0005829	cellular_component	cytosol
D	0008465	molecular_function	hydroxypyruvate reductase (NADH) activity
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0016618	molecular_function	hydroxypyruvate reductase [NAD(P)H] activity
D	0019752	biological_process	carboxylic acid metabolic process
D	0030267	molecular_function	glyoxylate reductase (NADPH) activity
D	0031406	molecular_function	carboxylic acid binding
D	0042803	molecular_function	protein homodimerization activity
D	0043648	biological_process	dicarboxylic acid metabolic process
D	0046487	biological_process	glyoxylate metabolic process
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
D	0070062	cellular_component	extracellular exosome
D	0070402	molecular_function	NADPH binding
D	0120509	molecular_function	hydroxypyruvate reductase (NADPH) activity
D	1902494	cellular_component	catalytic complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 1001

Chain	Residue
C	GLY150
C	THR152
C	GLN153
C	SER154
D	ARG300

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 1002

Chain	Residue
C	ASP37
D	LEU144
D	SO41003

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 D 1003

Chain	Residue
D	LEU144
D	TRP145
D	LEU146
D	SO41002
D	HOH2033
D	HOH2100
D	HOH2120
D	ARG125

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 1004

Chain	Residue
C	ARG300
D	GLY150
D	THR152
D	GLN153
D	SER154
D	HOH2067

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 1005

Chain	Residue
A	ARG300
B	GLY150
B	THR152
B	GLN153
B	SER154

site_id	AC6
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NDP A 2001

Chain	Residue
A	VAL83
A	THR111
A	ILE159
A	GLY160
A	LEU161
A	GLY162
A	ARG163
A	ILE164
A	GLY184
A	ARG185
A	GLN186
A	ARG188
A	CYS216
A	SER217
A	THR222
A	ILE243
A	SER244
A	ARG245
A	ASP269
A	HIS293
A	GLY295
A	SER296
A	DGY3001
A	HOH3003
A	HOH3012
A	HOH3021
A	HOH3030
A	HOH3169

site_id	AC7
Number of Residues	31
Details	BINDING SITE FOR RESIDUE NDP B 2002

Chain	Residue
B	VAL83
B	THR111
B	ILE159
B	GLY160
B	LEU161
B	GLY162
B	ARG163
B	ILE164
B	THR183
B	GLY184
B	ARG185
B	GLN186
B	ARG188
B	ALA215
B	CYS216
B	SER217
B	THR222
B	ILE243
B	SER244
B	ARG245
B	ASP269
B	HIS293
B	GLY295
B	SER296
B	HOH2008
B	HOH2013
B	HOH2023
B	HOH2030
B	HOH2035
B	HOH2043
B	HOH2045

site_id	AC8
Number of Residues	29
Details	BINDING SITE FOR RESIDUE NDP C 2003

Chain	Residue
C	SER217
C	THR222
C	ILE243
C	SER244
C	ARG245
C	ASP269
C	HIS293
C	GLY295
C	SER296
C	DGY3002
C	HOH3008
C	HOH3012
C	HOH3020
C	HOH3022
C	HOH3040
C	HOH3078
C	VAL83
C	THR111
C	ILE159
C	GLY160
C	GLY162
C	ARG163
C	ILE164
C	THR183
C	GLY184
C	ARG185
C	ARG188
C	ALA215
C	CYS216

site_id	AC9
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NDP D 2004

Chain	Residue
D	VAL83
D	THR111
D	GLY162
D	ARG163
D	ILE164
D	GLY184
D	ARG185
D	ARG188
D	ALA215
D	CYS216
D	SER217
D	THR222
D	ILE243
D	SER244
D	ARG245
D	ASP269
D	HIS293
D	GLY295
D	SER296
D	HOH2014
D	HOH2096
D	HOH2104
D	HOH2107

site_id	BC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE DGY A 3001

Chain	Residue
A	LEU59
A	SER82
A	VAL83
A	GLY84
A	LEU107
A	ARG245
A	HIS293
A	SER296
A	NDP2001
B	TRP141

site_id	BC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE DGY C 3002

Chain	Residue
C	LEU59
C	SER82
C	VAL83
C	GLY84
C	LEU107
C	ARG245
C	HIS293
C	SER296
C	NDP2003
D	TRP141

Functional Information from PROSITE/UniProt

site_id	PS00671
Number of Residues	17
Details	D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. MKeTaVFINiSRGdVVN

Chain	Residue	Details
A	MET234-ASN250

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"16756993","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	56
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16756993","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Site: {"description":"Raises pKa of active site His","evidences":[{"source":"PubMed","id":"16756993","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
A	ARG245
A	ASP269
A	GLU274
A	HIS293
A	ASP247

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
B	ARG245
B	ASP269
B	GLU274
B	HIS293
B	ASP247

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
C	ARG245
C	ASP269
C	GLU274
C	HIS293
C	ASP247

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
D	ARG245
D	ASP269
D	GLU274
D	HIS293
D	ASP247

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
A	GLU274
A	HIS293

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
B	GLU274
B	HIS293

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
C	GLU274
C	HIS293

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1gdh

Chain	Residue	Details
D	GLU274
D	HIS293

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)