Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2FSS

Candida boidinii formate dehydrogenase (FDH) K47E mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006734	biological_process	NADH metabolic process
A	0006735	biological_process	NADH regeneration
A	0008863	molecular_function	formate dehydrogenase (NAD+) activity
A	0015946	biological_process	methanol oxidation
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
A	0030416	biological_process	methylamine metabolic process
A	0042183	biological_process	formate catabolic process
A	0042426	biological_process	choline catabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0051287	molecular_function	NAD binding
A	0070403	molecular_function	NAD+ binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006734	biological_process	NADH metabolic process
B	0006735	biological_process	NADH regeneration
B	0008863	molecular_function	formate dehydrogenase (NAD+) activity
B	0015946	biological_process	methanol oxidation
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019752	biological_process	carboxylic acid metabolic process
B	0030416	biological_process	methylamine metabolic process
B	0042183	biological_process	formate catabolic process
B	0042426	biological_process	choline catabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0051287	molecular_function	NAD binding
B	0070403	molecular_function	NAD+ binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0006734	biological_process	NADH metabolic process
C	0006735	biological_process	NADH regeneration
C	0008863	molecular_function	formate dehydrogenase (NAD+) activity
C	0015946	biological_process	methanol oxidation
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
C	0030416	biological_process	methylamine metabolic process
C	0042183	biological_process	formate catabolic process
C	0042426	biological_process	choline catabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0051287	molecular_function	NAD binding
C	0070403	molecular_function	NAD+ binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006734	biological_process	NADH metabolic process
D	0006735	biological_process	NADH regeneration
D	0008863	molecular_function	formate dehydrogenase (NAD+) activity
D	0015946	biological_process	methanol oxidation
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019752	biological_process	carboxylic acid metabolic process
D	0030416	biological_process	methylamine metabolic process
D	0042183	biological_process	formate catabolic process
D	0042426	biological_process	choline catabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0051287	molecular_function	NAD binding
D	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 2001

Chain	Residue
D	GLY173
D	ARG174
D	HOH2018

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 2002

Chain	Residue
A	LYS109
C	GLN197
C	ALA198
C	ARG211
C	HOH2034
C	HOH2192

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 2003

Chain	Residue
A	ALA198
A	ARG211
A	HOH2021
C	LYS109

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 2004

Chain	Residue
C	GLY173
C	ARG174
C	HOH2047

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 2005

Chain	Residue
A	GLY173
A	ARG174
A	HOH2056
A	HOH2199

Functional Information from PROSITE/UniProt

site_id	PS00065
Number of Residues	29
Details	D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. IATIGaGRIGyrvlerlvpfnpkeLLyYD

Chain	Residue	Details
A	ILE167-ASP195

site_id	PS00670
Number of Residues	23
Details	D_2_HYDROXYACID_DH_2 D-isomer specific 2-hydroxyacid dehydrogenases signature 2. LVaqADIVtVNaPlhagTkgLiN

Chain	Residue	Details
A	LEU218-ASN240

site_id	PS00671
Number of Residues	17
Details	D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. FKkGaWLVNtARGaICV

Chain	Residue	Details
A	PHE247-VAL263

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_03210

Chain	Residue	Details
B	PRO230
B	THR256
B	ASP282
B	HIS311
C	VAL93
C	ASN119
C	ARG174
C	ASP195
C	PRO230
C	THR256
C	ASP282
C	HIS311
D	VAL93
D	ASN119
D	ARG174
D	ASP195
D	PRO230
D	THR256
D	ASP282
D	HIS311
A	VAL93
A	ASN119
A	ARG174
A	ASP195
A	PRO230
A	THR256
A	ASP282
A	HIS311
B	VAL93
B	ASN119
B	ARG174
B	ASP195

site_id	SWS_FT_FI2
Number of Residues	8
Details	SITE: Important for catalytic activity => ECO:0000255\|HAMAP-Rule:MF_03210, ECO:0000305\|PubMed:17525463

Chain	Residue	Details
C	ARG258
C	HIS311
D	ARG258
D	HIS311
A	ARG258
A	HIS311
B	ARG258
B	HIS311

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)