2FSS
Candida boidinii formate dehydrogenase (FDH) K47E mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008863 | molecular_function | formate dehydrogenase (NAD+) activity |
| A | 0015946 | biological_process | methanol oxidation |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0030416 | biological_process | methylamine metabolic process |
| A | 0042183 | biological_process | formate catabolic process |
| A | 0042426 | biological_process | choline catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0051287 | molecular_function | NAD binding |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008863 | molecular_function | formate dehydrogenase (NAD+) activity |
| B | 0015946 | biological_process | methanol oxidation |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0030416 | biological_process | methylamine metabolic process |
| B | 0042183 | biological_process | formate catabolic process |
| B | 0042426 | biological_process | choline catabolic process |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0051287 | molecular_function | NAD binding |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008863 | molecular_function | formate dehydrogenase (NAD+) activity |
| C | 0015946 | biological_process | methanol oxidation |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0030416 | biological_process | methylamine metabolic process |
| C | 0042183 | biological_process | formate catabolic process |
| C | 0042426 | biological_process | choline catabolic process |
| C | 0042803 | molecular_function | protein homodimerization activity |
| C | 0051287 | molecular_function | NAD binding |
| C | 0070403 | molecular_function | NAD+ binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0008863 | molecular_function | formate dehydrogenase (NAD+) activity |
| D | 0015946 | biological_process | methanol oxidation |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0030416 | biological_process | methylamine metabolic process |
| D | 0042183 | biological_process | formate catabolic process |
| D | 0042426 | biological_process | choline catabolic process |
| D | 0042803 | molecular_function | protein homodimerization activity |
| D | 0051287 | molecular_function | NAD binding |
| D | 0070403 | molecular_function | NAD+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 2001 |
| Chain | Residue |
| D | GLY173 |
| D | ARG174 |
| D | HOH2018 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 2002 |
| Chain | Residue |
| A | LYS109 |
| C | GLN197 |
| C | ALA198 |
| C | ARG211 |
| C | HOH2034 |
| C | HOH2192 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 2003 |
| Chain | Residue |
| A | ALA198 |
| A | ARG211 |
| A | HOH2021 |
| C | LYS109 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 2004 |
| Chain | Residue |
| C | GLY173 |
| C | ARG174 |
| C | HOH2047 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 2005 |
| Chain | Residue |
| A | GLY173 |
| A | ARG174 |
| A | HOH2056 |
| A | HOH2199 |
Functional Information from PROSITE/UniProt
| site_id | PS00065 |
| Number of Residues | 29 |
| Details | D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. IATIGaGRIGyrvlerlvpfnpkeLLyYD |
| Chain | Residue | Details |
| A | ILE167-ASP195 |
| site_id | PS00670 |
| Number of Residues | 23 |
| Details | D_2_HYDROXYACID_DH_2 D-isomer specific 2-hydroxyacid dehydrogenases signature 2. LVaqADIVtVNaPlhagTkgLiN |
| Chain | Residue | Details |
| A | LEU218-ASN240 |
| site_id | PS00671 |
| Number of Residues | 17 |
| Details | D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. FKkGaWLVNtARGaICV |
| Chain | Residue | Details |
| A | PHE247-VAL263 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 52 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03210","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Site: {"description":"Important for catalytic activity","evidences":[{"source":"HAMAP-Rule","id":"MF_03210","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17525463","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2nac |
| Chain | Residue | Details |
| A | HIS311 | |
| A | GLN287 | |
| A | ASN119 | |
| A | ARG258 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2nac |
| Chain | Residue | Details |
| B | HIS311 | |
| B | GLN287 | |
| B | ASN119 | |
| B | ARG258 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2nac |
| Chain | Residue | Details |
| C | HIS311 | |
| C | GLN287 | |
| C | ASN119 | |
| C | ARG258 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2nac |
| Chain | Residue | Details |
| D | HIS311 | |
| D | GLN287 | |
| D | ASN119 | |
| D | ARG258 |
