2FSS
Candida boidinii formate dehydrogenase (FDH) K47E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84140 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.430, 68.389, 109.369 |
| Unit cell angles | 77.93, 89.33, 81.34 |
Refinement procedure
| Resolution | 19.420 - 1.700 |
| R-factor | 0.20602 |
| Rwork | 0.204 |
| R-free | 0.24881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.410 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.850 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 158925 | |
| Completeness [%] | 96.5 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1M Na-Cacodylate pH 6.5, 30% PEG 8000, 0.15M Ammoniumsulfate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
