2E2K

Helicobacter pylori formamidase AmiF contains a fine-tuned cysteine-glutamate-lysine catalytic triad

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004328	molecular_function	formamidase activity
A	0006807	biological_process	obsolete nitrogen compound metabolic process
A	0016787	molecular_function	hydrolase activity
A	0033388	biological_process	putrescine biosynthetic process from arginine
A	0050126	molecular_function	N-carbamoylputrescine amidase activity
B	0004328	molecular_function	formamidase activity
B	0006807	biological_process	obsolete nitrogen compound metabolic process
B	0016787	molecular_function	hydrolase activity
B	0033388	biological_process	putrescine biosynthetic process from arginine
B	0050126	molecular_function	N-carbamoylputrescine amidase activity
C	0004328	molecular_function	formamidase activity
C	0006807	biological_process	obsolete nitrogen compound metabolic process
C	0016787	molecular_function	hydrolase activity
C	0033388	biological_process	putrescine biosynthetic process from arginine
C	0050126	molecular_function	N-carbamoylputrescine amidase activity
D	0004328	molecular_function	formamidase activity
D	0006807	biological_process	obsolete nitrogen compound metabolic process
D	0016787	molecular_function	hydrolase activity
D	0033388	biological_process	putrescine biosynthetic process from arginine
D	0050126	molecular_function	N-carbamoylputrescine amidase activity
E	0004328	molecular_function	formamidase activity
E	0006807	biological_process	obsolete nitrogen compound metabolic process
E	0016787	molecular_function	hydrolase activity
E	0033388	biological_process	putrescine biosynthetic process from arginine
E	0050126	molecular_function	N-carbamoylputrescine amidase activity
F	0004328	molecular_function	formamidase activity
F	0006807	biological_process	obsolete nitrogen compound metabolic process
F	0016787	molecular_function	hydrolase activity
F	0033388	biological_process	putrescine biosynthetic process from arginine
F	0050126	molecular_function	N-carbamoylputrescine amidase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton acceptor => ECO:0000250

Chain	Residue	Details
A	GLU60
B	GLU60
C	GLU60
D	GLU60
E	GLU60
F	GLU60

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site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Proton donor => ECO:0000250

Chain	Residue	Details
A	LYS133
B	LYS133
C	LYS133
D	LYS133
E	LYS133
F	LYS133

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site_id	SWS_FT_FI3
Number of Residues	6
Details	ACT_SITE: Nucleophile => ECO:0000250

Chain	Residue	Details
A	SER166
B	SER166
C	SER166
D	SER166
E	SER166
F	SER166

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