2E2K
Helicobacter pylori formamidase AmiF contains a fine-tuned cysteine-glutamate-lysine catalytic triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-05-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.717, 130.527, 144.589 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2576 |
| Rwork | 0.256 |
| R-free | 0.29378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 70795 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.2M lithium sulfate, 0.1M sodium acetate, 14% PEG 2000 MME, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
