2DD4

Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus recombinant apo-enzyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016787	molecular_function	hydrolase activity
A	0018760	molecular_function	thiocyanate hydrolase activity
A	0046265	biological_process	thiocyanate catabolic process
B	0016787	molecular_function	hydrolase activity
B	0018760	molecular_function	thiocyanate hydrolase activity
B	0046265	biological_process	thiocyanate catabolic process
C	0003824	molecular_function	catalytic activity
C	0016787	molecular_function	hydrolase activity
C	0018760	molecular_function	thiocyanate hydrolase activity
C	0046265	biological_process	thiocyanate catabolic process
C	0046872	molecular_function	metal ion binding
C	0046914	molecular_function	transition metal ion binding
D	0016787	molecular_function	hydrolase activity
D	0018760	molecular_function	thiocyanate hydrolase activity
D	0046265	biological_process	thiocyanate catabolic process
E	0016787	molecular_function	hydrolase activity
E	0018760	molecular_function	thiocyanate hydrolase activity
E	0046265	biological_process	thiocyanate catabolic process
F	0003824	molecular_function	catalytic activity
F	0016787	molecular_function	hydrolase activity
F	0018760	molecular_function	thiocyanate hydrolase activity
F	0046265	biological_process	thiocyanate catabolic process
F	0046872	molecular_function	metal ion binding
F	0046914	molecular_function	transition metal ion binding
G	0016787	molecular_function	hydrolase activity
G	0018760	molecular_function	thiocyanate hydrolase activity
G	0046265	biological_process	thiocyanate catabolic process
H	0016787	molecular_function	hydrolase activity
H	0018760	molecular_function	thiocyanate hydrolase activity
H	0046265	biological_process	thiocyanate catabolic process
I	0003824	molecular_function	catalytic activity
I	0016787	molecular_function	hydrolase activity
I	0018760	molecular_function	thiocyanate hydrolase activity
I	0046265	biological_process	thiocyanate catabolic process
I	0046872	molecular_function	metal ion binding
I	0046914	molecular_function	transition metal ion binding
J	0016787	molecular_function	hydrolase activity
J	0018760	molecular_function	thiocyanate hydrolase activity
J	0046265	biological_process	thiocyanate catabolic process
K	0016787	molecular_function	hydrolase activity
K	0018760	molecular_function	thiocyanate hydrolase activity
K	0046265	biological_process	thiocyanate catabolic process
L	0003824	molecular_function	catalytic activity
L	0016787	molecular_function	hydrolase activity
L	0018760	molecular_function	thiocyanate hydrolase activity
L	0046265	biological_process	thiocyanate catabolic process
L	0046872	molecular_function	metal ion binding
L	0046914	molecular_function	transition metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PDB","id":"2DD5","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Modified residue: {"description":"Cysteine sulfinic acid (-SO2H)","evidences":[{"source":"PubMed","id":"16417356","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16879822","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17222425","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"Cysteine sulfenic acid (-SOH)","evidences":[{"source":"PubMed","id":"16879822","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17222425","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ahj

Chain	Residue	Details
C	CYS133
C	CYS131
C	SER132
B	ARG91

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ahj

Chain	Residue	Details
F	CYS133
F	CYS131
F	SER132
E	ARG91

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1ahj

Chain	Residue	Details
H	ARG91
I	CYS133
I	CYS131
I	SER132

---

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1ahj

Chain	Residue	Details
K	ARG91
L	CYS133
L	CYS131
L	SER132

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 284

Chain	Residue	Details
C	CYS128	activator, electrostatic stabiliser, metal ligand
C	CYS131	electrostatic stabiliser, metal ligand
C	SER132	electrostatic stabiliser, metal ligand
C	CYS133	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, metal ligand

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 284

Chain	Residue	Details
F	CYS128	activator, electrostatic stabiliser, metal ligand
F	CYS131	electrostatic stabiliser, metal ligand
F	SER132	electrostatic stabiliser, metal ligand
F	CYS133	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, metal ligand

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 284

Chain	Residue	Details
I	CYS128	activator, electrostatic stabiliser, metal ligand
I	CYS131	electrostatic stabiliser, metal ligand
I	SER132	electrostatic stabiliser, metal ligand
I	CYS133	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, metal ligand

---

site_id	MCSA4
Number of Residues	4
Details	M-CSA 284

Chain	Residue	Details
L	CYS128	activator, electrostatic stabiliser, metal ligand
L	CYS131	electrostatic stabiliser, metal ligand
L	SER132	electrostatic stabiliser, metal ligand
L	CYS133	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, metal ligand

---