2DD4
Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus recombinant apo-enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 170.307, 175.602, 114.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.950 - 2.060 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.19300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 213749 | |
| Completeness [%] | 96.3 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5M Na/K Tartrate, 0.1M Potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5M Na/K Tartrate, 0.1M Potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
