2CF2

Architecture of mammalian fatty acid synthase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006633	biological_process	fatty acid biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0044281	biological_process	small molecule metabolic process
A	1903966	biological_process	monounsaturated fatty acid biosynthetic process
B	0016740	molecular_function	transferase activity
C	0005737	cellular_component	cytoplasm
C	0006633	biological_process	fatty acid biosynthetic process
C	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
D	0016491	molecular_function	oxidoreductase activity
J	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006629	biological_process	lipid metabolic process
J	0006631	biological_process	fatty acid metabolic process
J	0006633	biological_process	fatty acid biosynthetic process
J	0016740	molecular_function	transferase activity
J	0016746	molecular_function	acyltransferase activity
J	0044281	biological_process	small molecule metabolic process
J	1903966	biological_process	monounsaturated fatty acid biosynthetic process
K	0016740	molecular_function	transferase activity
L	0005737	cellular_component	cytoplasm
L	0006633	biological_process	fatty acid biosynthetic process
L	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
M	0016491	molecular_function	oxidoreductase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	804
Details	Domain: {"description":"Ketosynthase family 3 (KS3)","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"For beta-ketoacyl synthase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10571059","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"For beta-ketoacyl synthase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
C	VAL1076
C	GLY1079
C	HIS1070
C	CYS1080

---

site_id	CSA10
Number of Residues	6
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
J	PHE392
J	LYS328
J	HIS298
J	HIS333
J	PHE390
J	SER163

---

site_id	CSA11
Number of Residues	2
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
E	LYS155
E	TYR151

---

site_id	CSA12
Number of Residues	2
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
E	LYS155
E	GLN148

---

site_id	CSA13
Number of Residues	2
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
N	LYS155
N	GLN148

---

site_id	CSA14
Number of Residues	2
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
N	LYS155
N	TYR151

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
L	ASP1084

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
L	VAL1076
L	GLY1079
L	HIS1070
L	CYS1080

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
C	ASP1084

---

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
B	SER97
B	HIS201
B	GLN250

---

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
K	SER97
K	HIS201
K	GLN250

---

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
D	TYR49

---

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
M	TYR49

---

site_id	CSA9
Number of Residues	6
Details	Annotated By Reference To The Literature 1eq2

Chain	Residue	Details
A	PHE392
A	LYS328
A	HIS298
A	HIS333
A	PHE390
A	SER163

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 292

Chain	Residue	Details
A	SER163	activator, covalently attached, electrostatic stabiliser, hydrogen bond donor, nucleofuge, nucleophile
A	HIS298	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	LYS328	activator, hydrogen bond donor
A	HIS333	electrostatic stabiliser, hydrogen bond donor, increase basicity
A	PHE390	activator, hydrogen bond acceptor
A	PHE392	electrostatic stabiliser, hydrogen bond donor

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 292

Chain

Residue

Details

---