2BPO
Crystal structure of the yeast CPR triple mutant: D74G, Y75F, K78A.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| A | 0003959 | molecular_function | NADPH dehydrogenase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005741 | cellular_component | mitochondrial outer membrane |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006696 | biological_process | ergosterol biosynthetic process |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0010181 | molecular_function | FMN binding |
| A | 0016126 | biological_process | sterol biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0050661 | molecular_function | NADP binding |
| B | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| B | 0003959 | molecular_function | NADPH dehydrogenase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005741 | cellular_component | mitochondrial outer membrane |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006696 | biological_process | ergosterol biosynthetic process |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0010181 | molecular_function | FMN binding |
| B | 0016126 | biological_process | sterol biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 760 |
| Chain | Residue |
| A | SER282 |
| A | ASP284 |
| A | ARG285 |
| A | ASN582 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 761 |
| Chain | Residue |
| A | ASP626 |
| B | PRO337 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 B 762 |
| Chain | Residue |
| B | ARG553 |
| site_id | AC4 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE FAD A 750 |
| Chain | Residue |
| A | PRO365 |
| A | TYR405 |
| A | PHE406 |
| A | ASN407 |
| A | ARG439 |
| A | TYR440 |
| A | TYR441 |
| A | SER442 |
| A | THR457 |
| A | SER458 |
| A | ILE459 |
| A | GLU461 |
| A | PHE463 |
| A | VAL474 |
| A | VAL475 |
| A | GLY476 |
| A | VAL477 |
| A | THR478 |
| A | THR479 |
| A | TRP691 |
| A | FMN751 |
| A | NAP753 |
| A | HOH2073 |
| A | HOH2075 |
| A | HOH2123 |
| A | HOH2124 |
| A | HIS306 |
| A | GLY364 |
| site_id | AC5 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE FMN A 751 |
| Chain | Residue |
| A | SER67 |
| A | GLN68 |
| A | THR69 |
| A | GLY70 |
| A | THR71 |
| A | ALA72 |
| A | SER116 |
| A | THR117 |
| A | TYR118 |
| A | GLY119 |
| A | LEU152 |
| A | GLY153 |
| A | ASN154 |
| A | TYR157 |
| A | GLU158 |
| A | PHE159 |
| A | PHE160 |
| A | ASN161 |
| A | ASP187 |
| A | VAL690 |
| A | FAD750 |
| A | HOH2126 |
| A | HOH2127 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NAP A 753 |
| Chain | Residue |
| A | ARG285 |
| A | ILE459 |
| A | GLU461 |
| A | PRO541 |
| A | GLY542 |
| A | THR543 |
| A | SER580 |
| A | ARG581 |
| A | SER610 |
| A | ARG611 |
| A | LYS617 |
| A | TYR619 |
| A | GLN621 |
| A | ASP646 |
| A | ALA647 |
| A | LYS648 |
| A | GLY649 |
| A | MET650 |
| A | FAD750 |
| A | HOH2096 |
| A | HOH2101 |
| A | HOH2113 |
| site_id | AC7 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE FAD B 750 |
| Chain | Residue |
| B | THR479 |
| B | ARG522 |
| B | TRP691 |
| B | HOH2038 |
| B | HIS306 |
| B | GLY364 |
| B | PRO365 |
| B | TYR405 |
| B | PHE406 |
| B | ASN407 |
| B | ARG439 |
| B | TYR440 |
| B | TYR441 |
| B | SER442 |
| B | THR457 |
| B | SER458 |
| B | ILE459 |
| B | GLU461 |
| B | VAL474 |
| B | VAL475 |
| B | GLY476 |
| B | VAL477 |
| B | THR478 |
| site_id | AC8 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE FMN B 751 |
| Chain | Residue |
| B | SER67 |
| B | GLN68 |
| B | THR69 |
| B | GLY70 |
| B | THR71 |
| B | ALA72 |
| B | SER116 |
| B | THR117 |
| B | TYR118 |
| B | GLY119 |
| B | LEU152 |
| B | GLY153 |
| B | ASN154 |
| B | TYR157 |
| B | PHE159 |
| B | PHE160 |
| B | ASN161 |
| B | ASP187 |
| B | VAL690 |
| site_id | AC9 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE NAP B 753 |
| Chain | Residue |
| B | ARG285 |
| B | ILE459 |
| B | GLU461 |
| B | GLY542 |
| B | THR543 |
| B | SER580 |
| B | ARG581 |
| B | SER610 |
| B | ARG611 |
| B | LYS617 |
| B | TYR619 |
| B | GLN621 |
| B | ASP646 |
| B | ALA647 |
| B | LYS648 |
| B | GLY649 |
| B | MET650 |
| B | TRP691 |
| B | HOH2057 |
| B | HOH2058 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1332 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| A | PRO25-TRP691 | |
| B | PRO25-TRP691 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:16407065, ECO:0000269|PubMed:19483672 |
| Chain | Residue | Details |
| A | GLN68 | |
| A | THR117 | |
| A | GLY153 | |
| B | GLN68 | |
| B | THR117 | |
| B | GLY153 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:16407065 |
| Chain | Residue | Details |
| A | PHE79 | |
| A | ALA647 | |
| B | PHE79 | |
| B | ALA647 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 18 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:16407065 |
| Chain | Residue | Details |
| A | GLY188 | |
| B | GLY188 | |
| B | ASN286 | |
| B | TYR440 | |
| B | SER458 | |
| B | VAL477 | |
| B | GLY544 | |
| B | ARG611 | |
| B | VAL618 | |
| B | TRP691 | |
| A | ASN286 | |
| A | TYR440 | |
| A | SER458 | |
| A | VAL477 | |
| A | GLY544 | |
| A | ARG611 | |
| A | VAL618 | |
| A | TRP691 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| A | SER667 | |
| B | SER667 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ndh |
| Chain | Residue | Details |
| A | SER444 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ndh |
| Chain | Residue | Details |
| B | SER444 |
