Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2BKU

Kap95p:RanGTP complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0006913	biological_process	nucleocytoplasmic transport
B	0005085	molecular_function	guanyl-nucleotide exchange factor activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005635	cellular_component	nuclear envelope
B	0005643	cellular_component	nuclear pore
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006606	biological_process	protein import into nucleus
B	0006607	biological_process	NLS-bearing protein import into nucleus
B	0006612	biological_process	protein targeting to membrane
B	0006656	biological_process	phosphatidylcholine biosynthetic process
B	0006886	biological_process	intracellular protein transport
B	0008139	molecular_function	nuclear localization sequence binding
B	0015031	biological_process	protein transport
B	0031267	molecular_function	small GTPase binding
B	0042564	cellular_component	NLS-dependent protein nuclear import complex
B	0044877	molecular_function	protein-containing complex binding
B	0046822	biological_process	regulation of nucleocytoplasmic transport
B	0051028	biological_process	mRNA transport
B	0051170	biological_process	import into nucleus
B	0051292	biological_process	nuclear pore complex assembly
B	0060188	biological_process	regulation of protein desumoylation
B	0061608	molecular_function	nuclear import signal receptor activity
B	0061676	molecular_function	importin-alpha family protein binding
B	0097718	molecular_function	disordered domain specific binding
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0006913	biological_process	nucleocytoplasmic transport
D	0005085	molecular_function	guanyl-nucleotide exchange factor activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005635	cellular_component	nuclear envelope
D	0005643	cellular_component	nuclear pore
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006606	biological_process	protein import into nucleus
D	0006607	biological_process	NLS-bearing protein import into nucleus
D	0006612	biological_process	protein targeting to membrane
D	0006656	biological_process	phosphatidylcholine biosynthetic process
D	0006886	biological_process	intracellular protein transport
D	0008139	molecular_function	nuclear localization sequence binding
D	0015031	biological_process	protein transport
D	0031267	molecular_function	small GTPase binding
D	0042564	cellular_component	NLS-dependent protein nuclear import complex
D	0044877	molecular_function	protein-containing complex binding
D	0046822	biological_process	regulation of nucleocytoplasmic transport
D	0051028	biological_process	mRNA transport
D	0051170	biological_process	import into nucleus
D	0051292	biological_process	nuclear pore complex assembly
D	0060188	biological_process	regulation of protein desumoylation
D	0061608	molecular_function	nuclear import signal receptor activity
D	0061676	molecular_function	importin-alpha family protein binding
D	0097718	molecular_function	disordered domain specific binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 221

Chain	Residue
A	THR24
A	THR42
A	GTP220
A	HOH2002
A	HOH2004

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 221

Chain	Residue
C	HOH2003
C	THR24
C	THR42
C	GTP220
C	HOH2002

site_id	AC3
Number of Residues	22
Details	BINDING SITE FOR RESIDUE GTP A 220

Chain	Residue
A	GLY19
A	GLY20
A	THR21
A	GLY22
A	LYS23
A	THR24
A	THR25
A	PHE35
A	GLU36
A	LYS37
A	TYR39
A	THR42
A	GLY68
A	ASN122
A	LYS123
A	ASP125
A	ILE126
A	SER150
A	ALA151
A	LYS152
A	MG221
A	HOH2004

site_id	AC4
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GTP C 220

Chain	Residue
C	GLY19
C	GLY20
C	THR21
C	GLY22
C	LYS23
C	THR24
C	THR25
C	PHE35
C	GLU36
C	LYS37
C	TYR39
C	THR42
C	ALA67
C	GLY68
C	ASN122
C	LYS123
C	ASP125
C	ILE126
C	SER150
C	ALA151
C	LYS152
C	MG221
C	HOH2002
C	HOH2003

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Region: {"description":"Switch-I","evidences":[{"source":"PROSITE-ProRule","id":"PRU00752","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	32
Details	Region: {"description":"Switch-II","evidences":[{"source":"PROSITE-ProRule","id":"PRU00752","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15602554","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15864302","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19965479","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9533885","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9878368","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BYU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1WA5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2BKU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A6P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RAN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3W3Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5BXQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15602554","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15864302","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19965479","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1WA5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2BKU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3A6P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3W3Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15602554","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15864302","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19965479","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1WA5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2BKU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3W3Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Site: {"description":"Essential for GTP hydrolysis","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P62827","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P62826","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	64
Details	Repeat: {"description":"HEAT 1","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	162
Details	Domain: {"description":"Importin N-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00115","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	58
Details	Repeat: {"description":"HEAT 2","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	78
Details	Repeat: {"description":"HEAT 3","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	60
Details	Repeat: {"description":"HEAT 4","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	62
Details	Repeat: {"description":"HEAT 5","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	72
Details	Repeat: {"description":"HEAT 6","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	92
Details	Repeat: {"description":"HEAT 7","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	90
Details	Repeat: {"description":"HEAT 8","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	56
Details	Repeat: {"description":"HEAT 9","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	80
Details	Repeat: {"description":"HEAT 10","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	64
Details	Repeat: {"description":"HEAT 11","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	68
Details	Repeat: {"description":"HEAT 12","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	50
Details	Repeat: {"description":"HEAT 13","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	74
Details	Repeat: {"description":"HEAT 14","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	70
Details	Repeat: {"description":"HEAT 15","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	76
Details	Repeat: {"description":"HEAT 16","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI30
Number of Residues	92
Details	Repeat: {"description":"HEAT 17","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI31
Number of Residues	78
Details	Repeat: {"description":"HEAT 18","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI32
Number of Residues	80
Details	Repeat: {"description":"HEAT 19","evidences":[{"source":"PubMed","id":"15878174","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI33
Number of Residues	2
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI34
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	GLN69

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
C	GLN69

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)