2BKU

Kap95p:RanGTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	107.021, 127.879, 161.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.226
Rwork	0.223
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgk 1wa5
RMSD bond length	0.009
RMSD bond angle	1.093
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.070	0.380
Number of reflections	61240
<I/σ(I)>	10	2
Completeness [%]	99.5	99.7
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1