2BK2

The prepore structure of pneumolysin, obtained by fitting the alpha carbon trace of perfringolysin O into a cryo-EM map

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001897	biological_process	symbiont-mediated cytolysis of host cell
A	0005576	cellular_component	extracellular region
A	0008289	molecular_function	lipid binding
A	0015485	molecular_function	cholesterol binding
A	0016020	cellular_component	membrane
A	0020002	cellular_component	host cell plasma membrane
A	0031640	biological_process	killing of cells of another organism
A	0033644	cellular_component	host cell membrane
A	0035821	biological_process	modulation of process of another organism
A	0044179	biological_process	hemolysis in another organism
A	0090729	molecular_function	toxin activity

Functional Information from PROSITE/UniProt

site_id	PS00481
Number of Residues	12
Details	THIOL_CYTOLYSINS Thiol-activated cytolysins signature. RECTGLaWEWWR

Chain	Residue	Details
A	ARG457-ARG468

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	43
Details	TRANSMEM: Beta stranded => ECO:0000250|UniProtKB:Q04IN8

Chain	Residue	Details
A	VAL198-PHE211
A	GLU218-LYS227
A	ALA296-ASN305
A	TYR313-GLY325

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Important for cholesterol binding => ECO:0000305|PubMed:2888650

Chain	Residue	Details
A	ARG468

---