2BK2
The prepore structure of pneumolysin, obtained by fitting the alpha carbon trace of perfringolysin O into a cryo-EM map
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 135 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 28 |
| Symmetry | C31 |
