2BGN

HIV-1 Tat protein derived N-terminal nonapeptide Trp2-Tat(1-9) bound to the active site of Dipeptidyl peptidase IV (CD26)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001618	molecular_function	virus receptor activity
A	0001662	biological_process	behavioral fear response
A	0001666	biological_process	response to hypoxia
A	0002020	molecular_function	protease binding
A	0004177	molecular_function	aminopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005102	molecular_function	signaling receptor binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005765	cellular_component	lysosomal membrane
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0006508	biological_process	proteolysis
A	0007155	biological_process	cell adhesion
A	0008236	molecular_function	serine-type peptidase activity
A	0008239	molecular_function	dipeptidyl-peptidase activity
A	0008284	biological_process	positive regulation of cell population proliferation
A	0009986	cellular_component	cell surface
A	0010716	biological_process	negative regulation of extracellular matrix disassembly
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0016486	biological_process	peptide hormone processing
A	0019065	biological_process	receptor-mediated endocytosis of virus by host cell
A	0030027	cellular_component	lamellipodium
A	0030139	cellular_component	endocytic vesicle
A	0031258	cellular_component	lamellipodium membrane
A	0031295	biological_process	T cell costimulation
A	0033632	biological_process	regulation of cell-cell adhesion mediated by integrin
A	0035641	biological_process	locomotory exploration behavior
A	0036343	biological_process	psychomotor behavior
A	0042110	biological_process	T cell activation
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0042995	cellular_component	cell projection
A	0043542	biological_process	endothelial cell migration
A	0045121	cellular_component	membrane raft
A	0045499	molecular_function	chemorepellent activity
A	0046581	cellular_component	intercellular canaliculus
A	0046718	biological_process	symbiont entry into host cell
A	0046813	biological_process	receptor-mediated virion attachment to host cell
A	0050919	biological_process	negative chemotaxis
A	0061025	biological_process	membrane fusion
A	0070062	cellular_component	extracellular exosome
A	0070161	cellular_component	anchoring junction
A	0090024	biological_process	negative regulation of neutrophil chemotaxis
A	0120116	biological_process	glucagon processing
B	0001618	molecular_function	virus receptor activity
B	0001662	biological_process	behavioral fear response
B	0001666	biological_process	response to hypoxia
B	0002020	molecular_function	protease binding
B	0004177	molecular_function	aminopeptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005102	molecular_function	signaling receptor binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005765	cellular_component	lysosomal membrane
B	0005886	cellular_component	plasma membrane
B	0005925	cellular_component	focal adhesion
B	0006508	biological_process	proteolysis
B	0007155	biological_process	cell adhesion
B	0008236	molecular_function	serine-type peptidase activity
B	0008239	molecular_function	dipeptidyl-peptidase activity
B	0008284	biological_process	positive regulation of cell population proliferation
B	0009986	cellular_component	cell surface
B	0010716	biological_process	negative regulation of extracellular matrix disassembly
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0016486	biological_process	peptide hormone processing
B	0019065	biological_process	receptor-mediated endocytosis of virus by host cell
B	0030027	cellular_component	lamellipodium
B	0030139	cellular_component	endocytic vesicle
B	0031258	cellular_component	lamellipodium membrane
B	0031295	biological_process	T cell costimulation
B	0033632	biological_process	regulation of cell-cell adhesion mediated by integrin
B	0035641	biological_process	locomotory exploration behavior
B	0036343	biological_process	psychomotor behavior
B	0042110	biological_process	T cell activation
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0042995	cellular_component	cell projection
B	0043542	biological_process	endothelial cell migration
B	0045121	cellular_component	membrane raft
B	0045499	molecular_function	chemorepellent activity
B	0046581	cellular_component	intercellular canaliculus
B	0046718	biological_process	symbiont entry into host cell
B	0046813	biological_process	receptor-mediated virion attachment to host cell
B	0050919	biological_process	negative chemotaxis
B	0061025	biological_process	membrane fusion
B	0070062	cellular_component	extracellular exosome
B	0070161	cellular_component	anchoring junction
B	0090024	biological_process	negative regulation of neutrophil chemotaxis
B	0120116	biological_process	glucagon processing
C	0001618	molecular_function	virus receptor activity
C	0001662	biological_process	behavioral fear response
C	0001666	biological_process	response to hypoxia
C	0002020	molecular_function	protease binding
C	0004177	molecular_function	aminopeptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005102	molecular_function	signaling receptor binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005765	cellular_component	lysosomal membrane
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0006508	biological_process	proteolysis
C	0007155	biological_process	cell adhesion
C	0008236	molecular_function	serine-type peptidase activity
C	0008239	molecular_function	dipeptidyl-peptidase activity
C	0008284	biological_process	positive regulation of cell population proliferation
C	0009986	cellular_component	cell surface
C	0010716	biological_process	negative regulation of extracellular matrix disassembly
C	0016020	cellular_component	membrane
C	0016324	cellular_component	apical plasma membrane
C	0016486	biological_process	peptide hormone processing
C	0019065	biological_process	receptor-mediated endocytosis of virus by host cell
C	0030027	cellular_component	lamellipodium
C	0030139	cellular_component	endocytic vesicle
C	0031258	cellular_component	lamellipodium membrane
C	0031295	biological_process	T cell costimulation
C	0033632	biological_process	regulation of cell-cell adhesion mediated by integrin
C	0035641	biological_process	locomotory exploration behavior
C	0036343	biological_process	psychomotor behavior
C	0042110	biological_process	T cell activation
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0042995	cellular_component	cell projection
C	0043542	biological_process	endothelial cell migration
C	0045121	cellular_component	membrane raft
C	0045499	molecular_function	chemorepellent activity
C	0046581	cellular_component	intercellular canaliculus
C	0046718	biological_process	symbiont entry into host cell
C	0046813	biological_process	receptor-mediated virion attachment to host cell
C	0050919	biological_process	negative chemotaxis
C	0061025	biological_process	membrane fusion
C	0070062	cellular_component	extracellular exosome
C	0070161	cellular_component	anchoring junction
C	0090024	biological_process	negative regulation of neutrophil chemotaxis
C	0120116	biological_process	glucagon processing
D	0001618	molecular_function	virus receptor activity
D	0001662	biological_process	behavioral fear response
D	0001666	biological_process	response to hypoxia
D	0002020	molecular_function	protease binding
D	0004177	molecular_function	aminopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005102	molecular_function	signaling receptor binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005765	cellular_component	lysosomal membrane
D	0005886	cellular_component	plasma membrane
D	0005925	cellular_component	focal adhesion
D	0006508	biological_process	proteolysis
D	0007155	biological_process	cell adhesion
D	0008236	molecular_function	serine-type peptidase activity
D	0008239	molecular_function	dipeptidyl-peptidase activity
D	0008284	biological_process	positive regulation of cell population proliferation
D	0009986	cellular_component	cell surface
D	0010716	biological_process	negative regulation of extracellular matrix disassembly
D	0016020	cellular_component	membrane
D	0016324	cellular_component	apical plasma membrane
D	0016486	biological_process	peptide hormone processing
D	0019065	biological_process	receptor-mediated endocytosis of virus by host cell
D	0030027	cellular_component	lamellipodium
D	0030139	cellular_component	endocytic vesicle
D	0031258	cellular_component	lamellipodium membrane
D	0031295	biological_process	T cell costimulation
D	0033632	biological_process	regulation of cell-cell adhesion mediated by integrin
D	0035641	biological_process	locomotory exploration behavior
D	0036343	biological_process	psychomotor behavior
D	0042110	biological_process	T cell activation
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0042995	cellular_component	cell projection
D	0043542	biological_process	endothelial cell migration
D	0045121	cellular_component	membrane raft
D	0045499	molecular_function	chemorepellent activity
D	0046581	cellular_component	intercellular canaliculus
D	0046718	biological_process	symbiont entry into host cell
D	0046813	biological_process	receptor-mediated virion attachment to host cell
D	0050919	biological_process	negative chemotaxis
D	0061025	biological_process	membrane fusion
D	0070062	cellular_component	extracellular exosome
D	0070161	cellular_component	anchoring junction
D	0090024	biological_process	negative regulation of neutrophil chemotaxis
D	0120116	biological_process	glucagon processing
E	0004000	molecular_function	adenosine deaminase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005764	cellular_component	lysosome
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006154	biological_process	adenosine catabolic process
E	0007155	biological_process	cell adhesion
E	0008270	molecular_function	zinc ion binding
E	0009117	biological_process	nucleotide metabolic process
E	0009168	biological_process	purine ribonucleoside monophosphate biosynthetic process
E	0009897	cellular_component	external side of plasma membrane
E	0016787	molecular_function	hydrolase activity
E	0019239	molecular_function	deaminase activity
E	0031410	cellular_component	cytoplasmic vesicle
E	0042110	biological_process	T cell activation
E	0043103	biological_process	hypoxanthine salvage
E	0046103	biological_process	inosine biosynthetic process
E	0046872	molecular_function	metal ion binding
E	0046936	molecular_function	2'-deoxyadenosine deaminase activity
E	0060169	biological_process	negative regulation of adenosine receptor signaling pathway
E	0060205	cellular_component	cytoplasmic vesicle lumen
E	0070161	cellular_component	anchoring junction
F	0004000	molecular_function	adenosine deaminase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005764	cellular_component	lysosome
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006154	biological_process	adenosine catabolic process
F	0007155	biological_process	cell adhesion
F	0008270	molecular_function	zinc ion binding
F	0009117	biological_process	nucleotide metabolic process
F	0009168	biological_process	purine ribonucleoside monophosphate biosynthetic process
F	0009897	cellular_component	external side of plasma membrane
F	0016787	molecular_function	hydrolase activity
F	0019239	molecular_function	deaminase activity
F	0031410	cellular_component	cytoplasmic vesicle
F	0042110	biological_process	T cell activation
F	0043103	biological_process	hypoxanthine salvage
F	0046103	biological_process	inosine biosynthetic process
F	0046872	molecular_function	metal ion binding
F	0046936	molecular_function	2'-deoxyadenosine deaminase activity
F	0060169	biological_process	negative regulation of adenosine receptor signaling pathway
F	0060205	cellular_component	cytoplasmic vesicle lumen
F	0070161	cellular_component	anchoring junction
G	0004000	molecular_function	adenosine deaminase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005764	cellular_component	lysosome
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0006154	biological_process	adenosine catabolic process
G	0007155	biological_process	cell adhesion
G	0008270	molecular_function	zinc ion binding
G	0009117	biological_process	nucleotide metabolic process
G	0009168	biological_process	purine ribonucleoside monophosphate biosynthetic process
G	0009897	cellular_component	external side of plasma membrane
G	0016787	molecular_function	hydrolase activity
G	0019239	molecular_function	deaminase activity
G	0031410	cellular_component	cytoplasmic vesicle
G	0042110	biological_process	T cell activation
G	0043103	biological_process	hypoxanthine salvage
G	0046103	biological_process	inosine biosynthetic process
G	0046872	molecular_function	metal ion binding
G	0046936	molecular_function	2'-deoxyadenosine deaminase activity
G	0060169	biological_process	negative regulation of adenosine receptor signaling pathway
G	0060205	cellular_component	cytoplasmic vesicle lumen
G	0070161	cellular_component	anchoring junction
H	0004000	molecular_function	adenosine deaminase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005764	cellular_component	lysosome
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006154	biological_process	adenosine catabolic process
H	0007155	biological_process	cell adhesion
H	0008270	molecular_function	zinc ion binding
H	0009117	biological_process	nucleotide metabolic process
H	0009168	biological_process	purine ribonucleoside monophosphate biosynthetic process
H	0009897	cellular_component	external side of plasma membrane
H	0016787	molecular_function	hydrolase activity
H	0019239	molecular_function	deaminase activity
H	0031410	cellular_component	cytoplasmic vesicle
H	0042110	biological_process	T cell activation
H	0043103	biological_process	hypoxanthine salvage
H	0046103	biological_process	inosine biosynthetic process
H	0046872	molecular_function	metal ion binding
H	0046936	molecular_function	2'-deoxyadenosine deaminase activity
H	0060169	biological_process	negative regulation of adenosine receptor signaling pathway
H	0060205	cellular_component	cytoplasmic vesicle lumen
H	0070161	cellular_component	anchoring junction

Functional Information from PROSITE/UniProt

site_id	PS00708
Number of Residues	31
Details	PRO_ENDOPEP_SER Prolyl endopeptidase family serine active site. DqieAarqFskmgfvdnkriaiwGwSyGGYV

Chain	Residue	Details
A	ASP605-VAL635

---

site_id	PS00485
Number of Residues	7
Details	A_DEAMINASE Adenosine and AMP deaminase signature. SLNTDDP

Chain	Residue	Details
E	SER291-PRO297

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P03958

Chain	Residue	Details
E	VAL218
F	VAL218
G	VAL218
H	VAL218
B	ASP708
B	HIS740
C	SER630
C	ASP708
C	HIS740
D	SER630
D	ASP708
D	HIS740

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:12554940, ECO:0000269|PubMed:14709046, ECO:0000269|PubMed:15139750, ECO:0000269|PubMed:15213224, ECO:0000269|PubMed:15239652, ECO:0000269|PubMed:15695814, ECO:0000269|PubMed:16033254, ECO:0000269|PubMed:16060665, ECO:0000269|PubMed:18549808, ECO:0007744|PDB:1KRM, ECO:0007744|PDB:1NDV, ECO:0007744|PDB:1NDW, ECO:0007744|PDB:1NDY, ECO:0007744|PDB:1NDZ, ECO:0007744|PDB:1O5R, ECO:0007744|PDB:1QXL, ECO:0007744|PDB:1VFL

Chain	Residue	Details
E	VAL16
E	LEU18
E	ALA215
E	ASP296
F	VAL16
F	LEU18
F	ALA215
F	ASP296
G	VAL16
G	LEU18
G	ALA215
G	ASP296
H	VAL16
H	LEU18
H	ALA215
H	ASP296

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:12554940, ECO:0000269|PubMed:14709046, ECO:0000269|PubMed:15139750, ECO:0000269|PubMed:15239652, ECO:0000269|PubMed:16033254, ECO:0000269|PubMed:16060665, ECO:0000269|PubMed:18549808, ECO:0007744|PDB:1NDW, ECO:0007744|PDB:1NDY, ECO:0007744|PDB:1NDZ, ECO:0007744|PDB:1O5R, ECO:0007744|PDB:1QXL

Chain	Residue	Details
E	GLY20
F	GLY20
G	GLY20
H	GLY20

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:14709046, ECO:0000269|PubMed:16060665, ECO:0007744|PDB:1NDY, ECO:0007744|PDB:1WXY

Chain	Residue	Details
E	ASP185
F	ASP185
G	ASP185
H	ASP185

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:12554940, ECO:0000269|PubMed:14709046, ECO:0000269|PubMed:15139750, ECO:0000269|PubMed:15239652, ECO:0000269|PubMed:16033254, ECO:0000269|PubMed:18549808, ECO:0007744|PDB:1NDW, ECO:0007744|PDB:1NDY, ECO:0007744|PDB:1NDZ, ECO:0007744|PDB:1O5R, ECO:0007744|PDB:1QXL

Chain	Residue	Details
E	PRO297
F	PRO297
G	PRO297
H	PRO297

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	SITE: Important for interaction with adenosine receptors and increasing their affinity for agonists => ECO:0000250|UniProtKB:P00813

Chain	Residue	Details
E	PRO59
E	ALA63
F	PRO59
F	ALA63
G	PRO59
G	ALA63
H	PRO59
H	ALA63

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	SITE: Important for catalytic activity => ECO:0000250|UniProtKB:P03958

Chain	Residue	Details
E	GLY239
F	GLY239
G	GLY239
H	GLY239

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: N-acetylalanine => ECO:0000250|UniProtKB:P00813

Chain	Residue	Details
E	GLN3
F	GLN3
G	GLN3
H	GLN3

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P00813

Chain	Residue	Details
E	PRO55
E	THR233
F	PRO55
F	THR233
G	PRO55
G	THR233
H	PRO55
H	THR233

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 169

Chain	Residue	Details
A	TYR547	electrostatic stabiliser, hydrogen bond donor
A	SER630	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
A	TYR631	electrostatic stabiliser, hydrogen bond donor
A	ASP708	activator, electrostatic stabiliser, hydrogen bond acceptor
A	HIS740	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 169

Chain	Residue	Details
B	TYR547	electrostatic stabiliser, hydrogen bond donor
B	SER630	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
B	TYR631	electrostatic stabiliser, hydrogen bond donor
B	ASP708	activator, electrostatic stabiliser, hydrogen bond acceptor
B	HIS740	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---

site_id	MCSA3
Number of Residues	5
Details	M-CSA 169

Chain	Residue	Details
C	TYR547	electrostatic stabiliser, hydrogen bond donor
C	SER630	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
C	TYR631	electrostatic stabiliser, hydrogen bond donor
C	ASP708	activator, electrostatic stabiliser, hydrogen bond acceptor
C	HIS740	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---

site_id	MCSA4
Number of Residues	5
Details	M-CSA 169

Chain	Residue	Details
D	TYR547	electrostatic stabiliser, hydrogen bond donor
D	SER630	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
D	TYR631	electrostatic stabiliser, hydrogen bond donor
D	ASP708	activator, electrostatic stabiliser, hydrogen bond acceptor
D	HIS740	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---