2BGN
HIV-1 Tat protein derived N-terminal nonapeptide Trp2-Tat(1-9) bound to the active site of Dipeptidyl peptidase IV (CD26)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-07 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.945, 170.273, 239.200 |
Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.150 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w1i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.518 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.110 | 0.410 |
Number of reflections | 97782 | |
<I/σ(I)> | 9.7 | 2.6 |
Completeness [%] | 89.0 | 81 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |