2BC1

Structural Analysis of Streptococcus pyogenes NADH oxidase: C44S Nox

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0016491	molecular_function	oxidoreductase activity
A	1902600	biological_process	proton transmembrane transport
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0016491	molecular_function	oxidoreductase activity
B	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	36
Details	BINDING SITE FOR RESIDUE FAD A 457

Chain	Residue
A	VAL7
A	GLY43
A	SER44
A	MET46
A	PRO80
A	VAL81
A	ALA110
A	THR111
A	GLY112
A	SER113
A	VAL142
A	GLY8
A	LYS143
A	TYR170
A	ILE171
A	GLY291
A	ASP292
A	ALA308
A	LEU309
A	ALA310
A	SER311
A	HOH459
A	ASN10
A	HOH463
A	HOH464
A	HOH593
A	HOH609
B	PHE436
B	LEU437
B	HOH468
A	HIS11
A	ALA12
A	PHE33
A	ASP34
A	GLN35
A	ASN36

---

site_id	AC2
Number of Residues	36
Details	BINDING SITE FOR RESIDUE FAD B 457

Chain	Residue
A	PHE436
A	LEU437
B	VAL7
B	GLY8
B	ASN10
B	HIS11
B	ALA12
B	PHE33
B	ASP34
B	GLN35
B	GLY43
B	SER44
B	PRO80
B	VAL81
B	ALA110
B	THR111
B	GLY112
B	SER113
B	VAL142
B	LYS143
B	TYR170
B	ILE171
B	PHE255
B	GLY291
B	ASP292
B	ALA308
B	LEU309
B	ALA310
B	SER311
B	HOH461
B	HOH467
B	HOH469
B	HOH477
B	HOH486
B	HOH514
B	HOH564

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:P37062

Chain	Residue	Details
A	HIS11
B	HIS11

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Redox-active => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0

Chain	Residue	Details
A	SER44
B	SER44

---

site_id	SWS_FT_FI3
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0, ECO:0007744|PDB:2BC1, ECO:0007744|PDB:2BCP

Chain	Residue	Details
A	ASN10
A	ALA310
A	SER311
B	ASN10
B	ALA12
B	ASP34
B	GLN35
B	VAL81
B	SER113
B	LYS143
B	ASP292
A	ALA12
B	LEU309
B	ALA310
B	SER311
A	ASP34
A	GLN35
A	VAL81
A	SER113
A	LYS143
A	ASP292
A	LEU309

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0

Chain	Residue	Details
A	SER44
B	SER44

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0, ECO:0007744|PDB:2BCP

Chain	Residue	Details
A	ALA110
B	ALA110

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC1, ECO:0007744|PDB:2BCP

Chain	Residue	Details
A	TYR170
B	TYR170

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P37062

Chain	Residue	Details
A	ILE171
B	GLY254
B	ALA308
B	GLY339
A	ASP190
A	TYR199
A	GLY254
A	ALA308
A	GLY339
B	ILE171
B	ASP190
B	TYR199

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0, ECO:0007744|PDB:2BC1

Chain	Residue	Details
A	PHE436
B	PHE436

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Cysteine sulfinic acid (-SO2H) => ECO:0000269|PubMed:26506002, ECO:0007744|PDB:2BC0

Chain	Residue	Details
A	SER44
B	SER44

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1get

Chain	Residue	Details
A	HIS439
A	TYR444

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1get

Chain	Residue	Details
B	HIS439
B	TYR444

---