2BC1
Structural Analysis of Streptococcus pyogenes NADH oxidase: C44S Nox
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Detector | WEISSENBERG |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.614, 85.307, 148.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1JOA (NADH peroxidase poly-alanine) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.280 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.047 | 0.128 |
Number of reflections | 49891 | |
<I/σ(I)> | 17.8 | 8.3 |
Completeness [%] | 91.5 | 78.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | Ammonium sulfate, Na-K tartrate, Na citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |